KISS1 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

KISS1 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human KISS1.
  
  

  Source: E. coli

  Amino Acid Sequence: CTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRS

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: KISS1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-34010.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for KISS1 Recombinant Protein Antigen

• KiSS-1 metastasis-suppressor
• KiSS1
• KiSS-1
• kisspeptin
• Kisspeptin-1
• malignant melanoma metastasis-suppressor
• metastasis-suppressor KiSS-1
• Metastin
• MGC39258

Background

KISS1 gene encodes metastin, also known as kisspeptin or KiSS-1 peptide, which binds to a G protein-coupled receptor GPR54. KISS1 is dominantly expressed in brain (e.g. anteroventral periventricular nucleus, AVPV arcuate nucleus, ARC), placenta, and pancreas, etc. Metastin plays an important role in regulating the GnRH secretion in brain and MMP-9 expression in placenta, which is associated with development of puberty and sex maturation (e.g. Idiopathic hypogonadotropic hypogonadism, IHH) as well as placentation (e.g. pre-eclampsia), respectively. Metastin was originally discovered as a tumor metastasis suppressor (e.g. melanoma, breast cancer).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: KISS1