Human KISS1 ELISA Kit (Colorimetric)

ELISA: Human KISS1 ELISA Kit (Colorimetric) [NBP3-18751] - Samples were spiked with high concentrations of Human KISS1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human KISS1 ELISA Kit (Colorimetric) [NBP3-18751] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL

Human KISS1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human KISS1 in samples. No significant cross-reactivity or interference between Human KISS1 and analogues was observed.
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.88
• Intra-Assay: %CV < 5.88
• Spike Recovery: 87-98%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: KISS1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human KISS1 ELISA Kit (Colorimetric)

• KiSS-1 metastasis-suppressor
• KiSS1
• KiSS-1
• kisspeptin
• Kisspeptin-1
• malignant melanoma metastasis-suppressor
• metastasis-suppressor KiSS-1
• Metastin
• MGC39258

Background

KISS1 gene encodes metastin, also known as kisspeptin or KiSS-1 peptide, which binds to a G protein-coupled receptor GPR54. KISS1 is dominantly expressed in brain (e.g. anteroventral periventricular nucleus, AVPV arcuate nucleus, ARC), placenta, and pancreas, etc. Metastin plays an important role in regulating the GnRH secretion in brain and MMP-9 expression in placenta, which is associated with development of puberty and sex maturation (e.g. Idiopathic hypogonadotropic hypogonadism, IHH) as well as placentation (e.g. pre-eclampsia), respectively. Metastin was originally discovered as a tumor metastasis suppressor (e.g. melanoma, breast cancer).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for KISS1 ELISA Kit (NBP3-18751). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: KISS1