KIR2DL4/CD158d Antibody (mAb33)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IP, CyTOF-ready
Clone
mAb33
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

KIR2DL4/CD158d Antibody (mAb33) Summary

Immunogen
NK3.3 cells and KIR2DL4-Ig fusion protein
Specificity
The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KIR2DL4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1 ug/ml
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Functional Application: Cytokine secretion studies

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for KIR2DL4/CD158d Antibody (mAb33)

  • 103AS
  • 15.212
  • CD158 antigen-like family member D
  • CD158d antigen
  • CD158d
  • CD158DKIR103ASKiller cell inhibitory receptor 103AS
  • G9P
  • killer cell immunoglobulin-like receptor 2DL4
  • killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4
  • killer Ig receptor
  • KIR103
  • KIR-103AS
  • KIR2DL4
  • MHC class I NK cell receptor KIR103AS
  • natural killer cell inhibitory receptor
  • NK cell receptor

Background

CD158d / KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-gamma secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

PE Labeled NBP1-50067

Additional KIR2DL4/CD158d Products

Bioinformatics Tool for KIR2DL4/CD158d Antibody (NBP1-50052)

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Diseases for KIR2DL4/CD158d Antibody (NBP1-50052)

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Bioinformatics

Gene Symbol KIR2DL4
Entrez