Home » KIR2DL4/CD158d » KIR2DL4/CD158d Antibodies » KIR2DL4/CD158d Antibody (mAb33) [Alexa Fluor® 647]

KIR2DL4/CD158d Antibody (mAb33) [Alexa Fluor® 647]

 Submit a Review

Images

 
There are currently no images for KIR2DL4/CD158d Antibody (NBP1-50052AF647).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
mAb33
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-50052AF350
Alexa Fluor 405
NBP1-50052AF405
Alexa Fluor 488
NBP1-50052AF488
Alexa Fluor 532
NBP1-50052AF532
Alexa Fluor 594
NBP1-50052AF594
Alexa Fluor 700
NBP1-50052AF700
Alexa Fluor 750
NBP1-50052AF750
Allophycocyanin
NBP1-50052APC
Biotin
NBP1-50052B
DyLight 350
NBP1-50052UV
DyLight 405
NBP1-50052V
DyLight 488
NBP1-50052G
DyLight 550
NBP1-50052R
DyLight 594
NBP1-50052DL594
DyLight 650
NBP1-50052C
DyLight 680
NBP1-50052FR
DyLight 755
NBP1-50052IR
FITC
NBP1-50052F
HRP
NBP1-50052H
Janelia Fluor 549
NBP1-50052JF549
Janelia Fluor 646
NBP1-50052JF646
PE
NBP1-50052PE
PE
NBP1-50067
PerCP
NBP1-50052PCP
Unconjugated
NBP1-50052
100% Guarantee on every product

KIR2DL4/CD158d Antibody (mAb33) [Alexa Fluor® 647] Summary

Immunogen
NK3.3 cells and KIR2DL4-Ig fusion protein
Specificity
The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KIR2DL4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for KIR2DL4/CD158d Antibody (mAb33) [Alexa Fluor® 647]

  • 103AS
  • 15.212
  • CD158 antigen-like family member D
  • CD158d antigen
  • CD158d
  • CD158DKIR103ASKiller cell inhibitory receptor 103AS
  • G9P
  • killer cell immunoglobulin-like receptor 2DL4
  • killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4
  • killer Ig receptor
  • KIR103
  • KIR-103AS
  • KIR2DL4
  • MHC class I NK cell receptor KIR103AS
  • natural killer cell inhibitory receptor
  • NK cell receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

KIR3DL1 Antibody
(177407)
MAB12251
KIR3DL1 Antibody (177407)
(3 Publications)
Species: Hu
Applications: Flow, CyTOF-ready
GEM Antibody
NBP1-58906
Western Blot: GEM Antibody [NBP1-58906] - Human Stomach Tumor cell lysate. Antibody at 1.0 ug/mL.
Immunohistochemistry: GEM Antibody [NBP1-58906] - Human kidney tissue section. Magnification: 400X.
Western Blot: GEM Antibody [NBP1-58906] - HepG2 cell lysate. Antibody at 2.5 ug/mL.
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NKG2D/CD314
Antibody (149810) [Un ...
MAB139
Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
(1 Review)
(70 Publications)
Species: Hu
Applications: WB, Flow, CostimT, CyTOF-ready, Neut
NKG2A/CD159a/KLRC1
Antibody (131411) [Un ...
MAB1059
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2A/CD159a Monoclonal Antibody (Catalog # MAB1059) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
HEK293 Human embryonic kidney cell line transfected with either (A) Human NKG2A/CD159a, or (B) Human NKG2B/CD159b and eGFP was stained with Mouse Anti-Human NKG2A/CD159a Monoclonal Antibody (Catalog # MAB1059) followed by APC-conjugated anti-Mouse IgG secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.
(12 Publications)
Species: Hu
Applications: Flow, CyTOF-ready
CD94 Antibody
(131412) [Unconjugate
MAB1058
CD94 Antibody (131412) [Unconjugated]
(3 Publications)
Species: Hu
Applications: Flow, Block, CyTOF-ready
CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
(3 Reviews)
(1 Publication)
Species: Hu
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(20 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
HLA DQ Antibody
(SPV-L3)
NBP2-45041
Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
(1 Publication)
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
HLA B Antibody
(2G7B10)
NBP2-61871
Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of bladder cancer tissues using HLA-B mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
ILT3/CD85k/LILRB4
Antibody (293623) [Un ...
MAB24251
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL‑3 R alpha /CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human LILRB4/CD85k/ILT3 Monoclonal Antibody (Catalog # MAB24251) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). 
(2 Reviews)
(3 Publications)
Species: Hu
Applications: WB, Flow, CyTOF-ready
MHC Class I
Antibody (OX18)
NB120-6405
Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
(32 Publications)
Species: Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready, Flow-IC
KIR2DL3/CD158b2
Antibody (180701) [Un ...
MAB2014
Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL3/CD158b2 Monoclonal Antibody (Catalog # MAB2014) or (B) Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P).View our protocol for Staining Membrane-associated Proteins.
(1 Review)
(8 Publications)
Species: Hu
Applications: Flow, CyTOF-reported
IL-2 [Unconjugated]
202-IL
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
(22 Reviews)
(302 Publications)
Species: Hu
NKp46/NCR1
Antibody [Unconjugate
AF2225
Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody induces IFN-gamma secretion in mouse natural killer (NK) cells activated with 25 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) and 25 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML), in a dose-dependent manner, as measured using the Quantikine Mouse IFN-gamma ELISA Kit (Catalog # MIF00). The ED50 for this effect is typically0.4-2.4 μg/mL.
NKp46/NCR1 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Mouse DX5/CD49b+ splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2225, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
(2 Reviews)
(27 Publications)
Species: Mu
Applications: WB, Flow, IHC, AgAct, CyTOF-ready
HLA G Antibody
(87G)
NBP1-43123
Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - An intracellular stain was performed on Hek293 cells with HLA G Antibody [87G] NBP1-43123AF488 (blue) and a matched isotype control (orange). Both antibodies were conjugated to Alexa Fluor 488. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature.
Immunocytochemistry/Immunofluorescence: HLA G Antibody (87G) [NBP1-43123] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti HLA G (87G) at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - An intracellular stain was performed on HeLa cells with HLA G [87G] Antibody NBP1-43123AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
(8 Publications)
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, B/N, CyTOF-ready, Flow-CS
KIR2DL1/KIR2DS5
Antibody (143211) [Un ...
MAB1844
Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL1/KIR2DS5 Monoclonal Antibody (Catalog # MAB1844) or (B) Mouse IgG1 Isotype Control Antibody (Catalog # MAB002) followed by Anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.
(12 Publications)
Species: Hu
Applications: Flow, CyTOF-reported
HLA C Antibody
(DT-9)
NBP2-50419
HLA C Antibody (DT-9)
(5 Publications)
Species: Hu
Applications: Flow, IP, CyTOF-ready
CD161 Antibody
(14F1F11)
NBP2-14845
Western Blot: CD161 Antibody (14F1F11) [NBP2-14845] - Analysis of a partial CD161 recombinant protein using CD161 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: CD161 Antibody (14F1F11) [NBP2-14845] - Analysis using Azide Free version of NBP2-14845. CD161 in human kidney using CD161 antibody at 5 ug/ml.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
IFN-gamma
[Unconjugated]
285-IF
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma.
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
(19 Reviews)
(335 Publications)
Species: Hu
KIR2DL4/CD158d
Antibody (mAb33) [Ale ...
NBP1-50052AF647
KIR2DL4/CD158d Antibody (mAb33) [Alexa Fluor® 647]
Species: Hu
Applications: WB, Flow, ICC/IF

Publications for KIR2DL4/CD158d Antibody (NBP1-50052AF647) (0)

There are no publications for KIR2DL4/CD158d Antibody (NBP1-50052AF647).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for KIR2DL4/CD158d Antibody (NBP1-50052AF647) (0)

There are no reviews for KIR2DL4/CD158d Antibody (NBP1-50052AF647). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KIR2DL4/CD158d Antibody (NBP1-50052AF647) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-50052AF350
Alexa Fluor 405 NBP1-50052AF405
Alexa Fluor 488 NBP1-50052AF488
Alexa Fluor 532 NBP1-50052AF532
Alexa Fluor 594 NBP1-50052AF594
Alexa Fluor 647 NBP1-50052AF647
Alexa Fluor 700 NBP1-50052AF700
Alexa Fluor 750 NBP1-50052AF750
Allophycocyanin NBP1-50052APC
Biotin NBP1-50052B
DyLight 350 NBP1-50052UV
DyLight 405 NBP1-50052V
DyLight 488 NBP1-50052G
DyLight 550 NBP1-50052R
DyLight 594 NBP1-50052DL594
DyLight 650 NBP1-50052C
DyLight 680 NBP1-50052FR
DyLight 755 NBP1-50052IR
FITC NBP1-50052F
HRP NBP1-50052H
Janelia Fluor 549 NBP1-50052JF549
Janelia Fluor 646 NBP1-50052JF646
PE NBP1-50052PE
PE NBP1-50067
PerCP NBP1-50052PCP
Learn about Custom Antibody Labeling

Additional KIR2DL4/CD158d Products

Bioinformatics Tool for KIR2DL4/CD158d Antibody (NBP1-50052AF647)

Discover related pathways, diseases and genes to KIR2DL4/CD158d Antibody (NBP1-50052AF647). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for KIR2DL4/CD158d Antibody (NBP1-50052AF647)

Discover more about diseases related to KIR2DL4/CD158d Antibody (NBP1-50052AF647).
 

Pathways for KIR2DL4/CD158d Antibody (NBP1-50052AF647)

View related products by pathway.

PTMs for KIR2DL4/CD158d Antibody (NBP1-50052AF647)

Learn more about PTMs related to KIR2DL4/CD158d Antibody (NBP1-50052AF647).

Blogs on KIR2DL4/CD158d

There are no specific blogs for KIR2DL4/CD158d, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Mouse IgG1 Isotype Control (11711) [Alexa Fluor® 647]
IC002R
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma.
IFN-gamma [Unconjugated]
285-IF-100
KIR2DL4/CD158d
2238-KR-050
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
IL-2 [Unconjugated]
202-IL-010

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our KIR2DL4/CD158d Antibody (mAb33) [Alexa Fluor® 647] and receive a gift card or discount.


Bioinformatics

Gene Symbol KIR2DL4