KCNJ10 Antibody

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Western Blot: KCNJ10 Antibody [NBP1-20149] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:3000 incubated at 4C overnight.
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for ...read more
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. ...read more
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. ...read more
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of mouse kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin ...read more
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. ...read more
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. ...read more
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat heart. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. ...read more
Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

KCNJ10 Antibody Summary

Immunogen
A synthetic peptide from the c-terminal region of human KCNJ10 (Kir1.2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse and rat.
Specificity
KCNJ10.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KCNJ10
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:300-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Publications
Read Publication using
NBP1-20149 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for KCNJ10 Antibody

  • ATP-dependent inwardly rectifying potassium channel Kir4.1
  • ATP-sensitive inward rectifier potassium channel 10
  • BIRK-10
  • glial ATP-dependent inwardly rectifying potassium channel KIR4.1
  • Inward rectifier K(+) channel Kir1.2
  • inward rectifier K+ channel KIR1.2
  • KCNJ13-PEN
  • KIR1.2
  • KIR4.1
  • Potassium channel, inwardly rectifying subfamily J member 10
  • potassium inwardly-rectifying channel, subfamily J, member 10
  • SESAME

Background

FUNCTION: May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.; Subcellular location: Membrane, Multi-pass membrane protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KCNJ10 Antibody (NBP1-20149)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol KCNJ10
Entrez
Uniprot