KIR2.3 Antibody

Western Blot: KIR2.3 Antibody [NBP3-03005] - Analysis of extracts of various cell lines, using KIR2.3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit

Immunohistochemistry-Paraffin: KIR2.3 Antibody [NBP3-03005] - Paraffin-embedded rat brain using KIR2.3 antibody at dilution of 1:100 (40x lens).

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

KIR2.3 Antibody Summary

• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KIR2.3 (NP_004972.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNJ4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for KIR2.3 Antibody

• hippocampal inward rectifier potassium channel
• Hippocampal inward rectifier
• HIR
• HIRhIRK2
• HIRK2
• HRK1
• HRK1MGC142066
• Inward rectifier K(+) channel Kir2.3
• inward rectifier K+ channel Kir2.3
• inward rectifier potassium channel 4
• IRK3
• IRK-3
• IRK3MGC142068
• KCNJ4
• KIR2.3
• Potassium channel, inwardly rectifying subfamily J member 4
• potassium inwardly-rectifying channel, subfamily J, member 4

Background

The KIR2.3 (KCNJ4) gene codes for protein that is 445 amino acid long inward rectifier potassium channel that regulates channel flow critical for electrical signaling of the nervous system, allowing for muscle excitability and neural firing. The KCNJ4 has a mass of approximately 49 kDA. Researchers are investigating the role of the KIR2.3 gene in meniere's disease, neuronitis, hyperglycemia, and noma. This gene has been shown to interact with other genes such as LIN7B, CASK, DLG4, IL16, and DLG1 in pathways such as potassium transport (in and out), antiarrhythmic pathways, dopamine-DARPP32 feedback onto cAMP pathways, and in the cholinergic synapse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCNJ4