Kinesin 5B Antibody - BSA Free

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Western Blot: Kinesin Antibody [NB500-580] - Detection of kinesin in reduced lysates of human HEK cell line (1) and porcine brain (2) by rabbit polyclonal anti-kinesin.

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Kinesin 5B Antibody - BSA Free Summary

Immunogen
Stalk domain of human kinesin (amino acids 331-906) expressed in E. coli (FKHC3).
Specificity
This detects total level of endogenous kinesin protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIF5B
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:1000
Application Notes
Predicted molecular weight: 110 kDa. Purity is > 95% (by SDS-PAGE)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Affinity purified

Alternate Names for Kinesin 5B Antibody - BSA Free

  • Conventional kinesin heavy chain
  • kinesin family member 5B
  • kinesin-1 heavy chain
  • KNS1kinesin 1 (110-120kD)
  • KNSKINH
  • Ubiquitous kinesin heavy chain
  • UKHCkinesin heavy chain

Background

Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N terminal globular motordomain with both a microtubule binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KIF5B
Entrez