JNK1/2/3 [p Thr183, p Tyr185] Antibody [Unconjugated]

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line and NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 J/m2 UV-C for 30 minutes. PVDF membrane was probed with 0.5 ...read more
JNK phosphorylated at T183/Y185 was detected in immersion fixed paraffin-embedded sections of human prostate array using Rabbit Anti-Human/Mouse/Rat Phospho‑JNK (T183/Y185) Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100 J/m2 ultraviolet light (UV) for 30 minutes, loaded at 0.2 mg/mL. Specific bands were detected for ...read more
JNK phosphorylated at T183/Y185 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

JNK1/2/3 [p Thr183, p Tyr185] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human, rat, and mouse JNK1 T183/Y185 site
Modification
p Thr183, p Tyr185
Specificity
Detects human, mouse and rat p46 and p54 JNK when dually phosphorylated at sites homologous to T183/Y185 of JNK1 and JNK2, and T221/Y223 of JNK3 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK8IP3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 3
using
AF1205 in the following applications:

Publications
Read Publications using
AF1205 in the following applications:

  • WB
    9 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JNK1/2/3 [p Thr183, p Tyr185] Antibody [Unconjugated]

  • c-Jun N-terminal kinase 1
  • EC 2.7.11
  • EC 2.7.11.24
  • JNK
  • JNK1 alpha protein kinase
  • JNK1 beta protein kinase
  • JNK1JNK1A2
  • JNK-46
  • JUN N-terminal kinase
  • MAP kinase 8
  • MAPK 8
  • mitogen-activated protein kinase 8 isoform JNK1 alpha1
  • mitogen-activated protein kinase 8 isoform JNK1 beta2
  • mitogen-activated protein kinase 8
  • PRKM8JNK
  • protein kinase JNK1
  • SAPK1JNK21B1/2
  • Stress-activated protein kinase 1
  • Stress-activated protein kinase JNK1

Background

The c-Jun N-terminal Kinases (JNKs) are part of the MAPK (mitogen-activated protein kinase) system that transmits signals from the extracellular milieu to both the cytoplasm and nucleus of the cell. Following perturbation at the cell membrane, MEKKs/MAP3Ks are initially activated, followed by their activation of MKKs/MAP2Ks, and MKKs activation of MAPKs/MAP(1)Ks. There are three classes of MAPKs: ERKs, p38 Kinases and JNKs. JNKs are 45-55 kDa protein products of three genes which, through alternative splicing, generate up to 10 possible isoforms. The phosphorylation targets for MAPKs vary, but include p53, c-MYC, ATF2 and c-Jun, the latter molecule representing the namesake for the enzyme group. The three human JNKs share approximately 80% aa sequence identity. JNKs from human, mouse and rat all contain a conserved Met-Met-Thr(183)-Pro-Tyr(185)-Val-Val motif that undergoes dual phosphorylation by MMK4 and MMK7 to activate the different JNKs. Activated by environmental stresses and inflammatory cytokines, JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JNK1/2/3 Antibody (AF1205)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(9)
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Human
(6)
Mouse
(1)
Rat
(2)
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Showing Publications 1 - 9 of 9.
Publications using AF1205 Applications Species
S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response Cell Death Dis, 2017;8(7):e2955. 2017 [PMID: 28749468] (WB,, Human) WB Human
EM Mantawy, A Esmat, WM El-Bakly, RA Salah ElDi, E El-Demerda Mechanistic clues to the protective effect of chrysin against doxorubicin-induced cardiomyopathy: Plausible roles of p53, MAPK and AKT pathways Sci Rep, 2017;7(1):4795. 2017 [PMID: 28684738] (WB,, Rat) WB Rat
Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C White-to-brown metabolic conversion of human adipocytes by JAK inhibition. Nat Cell Biol, 2015;17(1):57-67. 2015 [PMID: 25487280] (WB,, Human) WB Human
Shin E, Huang Q, Gurel Z, Sorenson C, Sheibani N High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress. PLoS ONE, 2014;9(7):e103148. 2014 [PMID: 25068294] (WB,, Mouse) WB Mouse
Ashraf M, Ebner M, Wallner C, Haller M, Khalid S, Schwelberger H, Koziel K, Enthammer M, Hermann M, Sickinger S, Soleiman A, Steger C, Vallant S, Sucher R, Brandacher G, Santer P, Dragun D, Troppmair J A p38MAPK/MK2 signaling pathway leading to redox stress, cell death and ischemia/reperfusion injury. Cell Commun Signal, 2014;12(0):6. 2014 [PMID: 24423080] (WB,, Rat) WB Rat
Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes. J Biol Chem, 2012;287(32):26478-94. 2012 [PMID: 22707717] (WB,, Human) WB Human
Protective effect of the poly(ADP-ribose) polymerase inhibitor PJ34 on mitochondrial depolarization-mediated cell death in hepatocellular carcinoma cells involves attenuation of c-Jun N-terminal kinase-2 and protein kinase B/Akt activation. Mol. Cancer, 2012;11(0):34. 2012 [PMID: 22583868] (WB,, Human) WB Human
Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema. Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400. 2009 [PMID: 19525388] (WB,, Human) WB Human
Bengochea A, de Souza MM, Lefrancois L, Le Roux E, Galy O, Chemin I, Kim M, Wands JR, Trepo C, Hainaut P, Scoazec JY, Vitvitski L, Merle P Common dysregulation of Wnt/Frizzled receptor elements in human hepatocellular carcinoma. Br. J. Cancer, 2008;99(1):143-50. 2008 [PMID: 18577996] (WB,, Human) WB Human

Review for JNK1/2/3 Antibody (AF1205) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using AF1205:
Filter by Applications
WB
(1)
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Aleksandra Hac
WB Human and Mouse 05/04/2017
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Summary

ApplicationWestern Blot
Sample TestedMEF whole cell lysate,PC3 Whole Cell Lysate (Human prostate cancer cell),HDFa whole cell lysate
SpeciesHuman and Mouse
LotHCT0517011

Comments

CommentsWe obtained bands at predicted molecular weight for both human (PC3, HDFa) as well as mouse (MEF) samples. However, at recommended concentrations the signal was quite weak using ECL substrate. Perhaps they work better using substrates with higher sensitivity.

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Aleksandra Hac
05/04/2017
Application: WB
Species: Human and Mouse

Bioinformatics

Gene Symbol MAPK8IP3