JNK1/2/3 [p Thr183, p Tyr185] Antibody [Unconjugated]


Western blot shows lysates of HEK293 human embryonic kidney cell line and NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 J/m2 UV-C for 30 minutes. PVDF membrane was probed with 0.5 ...read more
JNK phosphorylated at T183/Y185 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal ...read more
JNK phosphorylated at T183/Y185 was detected in immersion fixed paraffin-embedded sections of human prostate array using Rabbit Anti-Human/Mouse/Rat Phospho‑JNK (T183/Y185) Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100 J/m2 ultraviolet light (UV) for 30 minutes, loaded at 0.2 mg/mL. Specific bands were detected for ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC

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JNK1/2/3 [p Thr183, p Tyr185] Antibody [Unconjugated] Summary

Phosphopeptide containing human, rat, and mouse JNK1 T183/Y185 site
p Thr183, p Tyr185
Detects human, mouse and rat p46 and p54 JNK when dually phosphorylated at sites homologous to T183/Y185 of JNK1 and JNK2, and T221/Y223 of JNK3 in Western blots.
Purity Statement
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 3
AF1205 in the following applications:

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AF1205 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JNK1/2/3 [p Thr183, p Tyr185] Antibody [Unconjugated]

  • c-Jun N-terminal kinase 1
  • EC 2.7.11
  • EC
  • JNK
  • JNK1 alpha protein kinase
  • JNK1 beta protein kinase
  • JNK1JNK1A2
  • JNK-46
  • JUN N-terminal kinase
  • MAP kinase 8
  • MAPK 8
  • mitogen-activated protein kinase 8 isoform JNK1 alpha1
  • mitogen-activated protein kinase 8 isoform JNK1 beta2
  • mitogen-activated protein kinase 8
  • protein kinase JNK1
  • SAPK1JNK21B1/2
  • Stress-activated protein kinase 1
  • Stress-activated protein kinase JNK1


The c-Jun N-terminal Kinases (JNKs) are part of the MAPK (mitogen-activated protein kinase) system that transmits signals from the extracellular milieu to both the cytoplasm and nucleus of the cell. Following perturbation at the cell membrane, MEKKs/MAP3Ks are initially activated, followed by their activation of MKKs/MAP2Ks, and MKKs activation of MAPKs/MAP(1)Ks. There are three classes of MAPKs: ERKs, p38 Kinases and JNKs. JNKs are 45-55 kDa protein products of three genes which, through alternative splicing, generate up to 10 possible isoforms. The phosphorylation targets for MAPKs vary, but include p53, c-MYC, ATF2 and c-Jun, the latter molecule representing the namesake for the enzyme group. The three human JNKs share approximately 80% aa sequence identity. JNKs from human, mouse and rat all contain a conserved Met-Met-Thr(183)-Pro-Tyr(185)-Val-Val motif that undergoes dual phosphorylation by MMK4 and MMK7 to activate the different JNKs. Activated by environmental stresses and inflammatory cytokines, JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JNK1/2/3 Antibody (AF1205)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IHC, Western Blot.

Filter By Application
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF1205 Applications Species
K Suyama, D Sakai, S Hayashi, N Qu, H Terayama, D Kiyoshima, K Nagahori, M Watanabe Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70 Biomedicines, 2023-03-12;11(3):. 2023-03-12 [PMID: 36979842] (Western Blot, Human) Western Blot Human
T Ishijima, K Nakajima Changes of signaling molecules in the axotomized rat facial nucleus Journal of chemical neuroanatomy, 2022-10-29;126(0):102179. 2022-10-29 [PMID: 36341893] (IHC, Rat) IHC Rat
EM Mantawy, RS Said, DH Kassem, AK Abdel-Aziz, AM Badr Novel molecular mechanisms underlying the ameliorative effect of N-acetyl-L-cysteine against ?-radiation-induced premature ovarian failure in rats Ecotoxicol. Environ. Saf., 2020-08-29;206(0):111190. 2020-08-29 [PMID: 32871518] (IHC, Rat) IHC Rat
J Falero-Per, N Sheibani, CM Sorenson Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells PLoS ONE, 2020-05-04;15(5):e0232779. 2020-05-04 [PMID: 32365083] (Western Blot, Mouse) Western Blot Mouse
JM Jiménez-Va, V Herrero-Ag, AJ Montero-Hi, E Gómez-Góme, AC Fuentes-Fa, AJ León-Gonzá, P Sáez-Martí, E Alors-Pére, S Pedraza-Ar, T González-S, O Reyes, A Martínez-L, R Sánchez-Sá, S Ventura, EM Yubero-Ser, MJ Requena-Ta, JP Castaño, MD Gahete, RM Luque Dysregulation of the splicing machinery is directly associated to aggressiveness of prostate cancer EBioMedicine, 2020-01-03;51(0):102547. 2020-01-03 [PMID: 31902674] (Western Blot, Human) Western Blot Human
K Suyama, D Sakai, N Hirayama, Y Nakamura, E Matsushita, H Terayama, N Qu, O Tanaka, K Sakabe, M Watanabe Effects of interleukin-17A in nucleus pulposus cells and its small-molecule inhibitors for intervertebral disc disease J. Cell. Mol. Med., 2018-09-11;0(0):. 2018-09-11 [PMID: 30207057] (Western Blot, Rat) Western Blot Rat
S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response Cell Death Dis, 2017-07-27;8(7):e2955. 2017-07-27 [PMID: 28749468] (Western Blot, Human) Western Blot Human
EM Mantawy, A Esmat, WM El-Bakly, RA Salah ElDi, E El-Demerda Mechanistic clues to the protective effect of chrysin against doxorubicin-induced cardiomyopathy: Plausible roles of p53, MAPK and AKT pathways Sci Rep, 2017-07-06;7(1):4795. 2017-07-06 [PMID: 28684738] (Western Blot, Rat) Western Blot Rat
Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C White-to-brown metabolic conversion of human adipocytes by JAK inhibition. Nat Cell Biol, 2014-12-08;17(1):57-67. 2014-12-08 [PMID: 25487280] (Western Blot, Human) Western Blot Human
Shin E, Huang Q, Gurel Z, Sorenson C, Sheibani N High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress. PLoS ONE, 2014-07-28;9(7):e103148. 2014-07-28 [PMID: 25068294] (Western Blot, Mouse) Western Blot Mouse
Show All 15 Publications.

Review for JNK1/2/3 Antibody (AF1205) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using AF1205:
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Images Ratings Applications Species Date Details
reviewed by:
Aleksandra Hac
WB Human and Mouse 05/04/2017


ApplicationWestern Blot
Sample TestedHDFa whole cell lysate,MEF whole cell lysate,PC3 Whole Cell Lysate (Human prostate cancer cell)
SpeciesHuman and Mouse


CommentsWe obtained bands at predicted molecular weight for both human (PC3, HDFa) as well as mouse (MEF) samples. However, at recommended concentrations the signal was quite weak using ECL substrate. Perhaps they work better using substrates with higher sensitivity.

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Recent Reviews


Aleksandra Hac
Application: WB
Species: Human and Mouse


Gene Symbol MAPK8IP3