KIF2C Antibody

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MCAK antibody [N1N3] (NBP2-17054) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

MCAK antibody [N1N3] detects MCAK protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MCAK protein stained by MCAK antibody [N1N3] (NBP2-17054) diluted at 1:500.Blue: Hoechst 33342 staining.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

KIF2C Antibody Summary

• Description: Novus Biologicals Rabbit KIF2C Antibody (NBP2-17054) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human KIF2C. The exact sequence is proprietary.
• Localization: Cytoplasm; cytoskeleton (By similarity); Nucleus (By similarity); Centromere
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF2C
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for KIF2C Antibody

• kinesin family member 2C
• kinesin-like 6 (mitotic centromere-associated kinesin)
• Kinesin-like protein 6
• kinesin-like protein KIF2C
• MCAKKNSL6kinesin-like 6
• Mitotic centromere-associated kinesin

Background

The protein encoded by this gene is a member of kinesin-like protein family. Proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: KIF2C
• Uniprot:
  • Q99661(Human)