KIF2C Antibody - BSA Free

Western Blot: KIF2C Antibody [NB100-2588] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCAK antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds..

Immunoprecipitation: KIF2C Antibody [NB100-2588] - Detection of human MCAK by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCAK antibody NB100-2588 used for IP at 3 ug per reaction. MCAK was also immunoprecipitated by rabbit anti-MCAK antibody NB100-2587. For blotting immunoprecipitated MCAK, NB100-2588 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

KIF2C Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit KIF2C Antibody - BSA Free (NB100-2588) is a polyclonal antibody validated for use in WB and IP. Anti-KIF2C Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human Mitotic Centromere-Associated Kinesin using the numbering given in entry NP_006836.1 (GeneID 11004).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF2C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  KIF2C Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in (PMID: 23055517).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KIF2C Antibody - BSA Free

• kinesin family member 2C
• kinesin-like 6 (mitotic centromere-associated kinesin)
• Kinesin-like protein 6
• kinesin-like protein KIF2C
• MCAKKNSL6kinesin-like 6
• Mitotic centromere-associated kinesin

Background

The protein encoded by this gene is a member of kinesin-like protein family. Proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIF2C Antibody (NB100-2588)(3)

Publications using NB100-2588

• Jörg Schweiggert, Gregor Habeck, Sandra Hess, Felix Mikus, Roman Beloshistov, Klaus Meese, Shoji Hata, Klaus‐Peter Knobeloch, Frauke Melchior SCF Fbxw5 targets kinesin‐13 proteins to facilitate ciliogenesis The EMBO Journal 2021-08-09 [PMID: 34368969]
• Drum BM, Yuan C, Li L et al. Oxidative stress decreases microtubule growth and stability in ventricular myocytes. J Mol Cell Cardiol 2016-04-01 [PMID: 26902968]
• Pakala SB, Nair VS, Reddy SD, Kumar R. Signaling-dependent phosphorylation of Mitotic Centromere Associated Kinesin regulates microtubule depolymerization and its centrosomal localization J Biol Chem 2012-10-10 [PMID: 23055517] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: KIF2C
• Entrez:
  • 11004(Human)
• Uniprot:
  • Q99661(Human)