Ki-67/MKI67 Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit

Ki-67/MKI67 Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to an internal region of human Ki67 (within residues 1550-1700) [Swiss-Prot# P46013]
Localization
Nuclear
Marker
Proliferation Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MKI67
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Biotin
Format
Biotin (A=N/A, E=N/A)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:20-1:50
  • Immunohistochemistry 1:50-1:300
  • Immunohistochemistry-Frozen 1:50-1:300
  • Immunohistochemistry-Paraffin 1:50-1:300
Application Notes
This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot; however, NB500-170 has been used successfully Western Blog reported by a customer review. *Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear).
Readout System

Reactivity Notes

Human, mouse, rat (pubmed 22521325) and porcine.

Alternate Names for Ki-67/MKI67 Antibody [Biotin]

  • antigen identified by monoclonal Ki-67
  • antigen KI-67
  • Ki67
  • Ki-67
  • Ki-67/MKI67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-1
  • MKI67
  • PPP1R105
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Background

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Ki-67/MKI67 Antibody (NB500-170B) (0)

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MKI67
Entrez
Uniprot

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