KF1 Antibody (3E7) - Azide and BSA Free

Western Blot: KF1 Antibody (3E7) [H00007844-M01] - Analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi ( Cat # H00007844-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 (Cat # H00007844-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: KF1 Antibody (3E7) [H00007844-M01] - Analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, Mycoplasma
• Clone: 3E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

KF1 Antibody (3E7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RNF103 (NP_005658, 501 a.a. ~ 599 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HPLIPTDYIKNLPMWRFKCLGVQSEEEMSEGSQDTENDSESENTDTLSSEKEVFEDKQSVLHNSPGTASHCDAEACSCANKYCQTSPCERKGRSYGSYN
• Specificity: RNF103 - ring finger protein 103
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNF103
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for KF1 Antibody (3E7) - Azide and BSA Free

• E3 ubiquitin-protein ligase RNF103
• EC 6.3.2.-
• hkf-1
• KF-1
• KF1ZFP103ZFP-103
• MGC102815
• ring finger protein 103MGC41857
• Zfp-103
• zinc finger protein 103 homolog (mouse)
• Zinc finger protein 103 homolog
• zinc finger protein expressed in cerebellum

Background

The protein encoded by this gene contains a RING-H2 finger, a motif known to be involved in protein-protein and protein-DNA interactions. This gene is highly expressed in normal cerebellum, but not in the cerebral cortex. The expression of the rat counterpart in the frontal cortex and hippocampus was shown to be induced by elctroconvulsive treatment (ECT) as well as chronic antidepressant treatment, suggesting that this gene may be a molecular target for ECT and antidepressants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF103
• Entrez:
  • 7844(Human)
• OMIM:
  • 602507(Human)
• Uniprot:
  • NP_005658(Human)