Human KERA ELISA Kit (Colorimetric) Summary
| Description |
Assay Length: 4.5 hours |
| Standard Curve Range |
0.312 - 20 ng/mL (example only; lot dependent) |
| Sensitivity |
0.107 ng/mL (example only; lot dependent) |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
%CV < 12 (example only; lot dependent) |
| Intra-Assay |
%CV < 10 (example only; lot dependent) |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
KERA |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Detection Reagent A
- Detection Reagent B
- Diluent Buffer
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
|
Alternate Names for Human KERA ELISA Kit (Colorimetric)
Background
KERA is a gene that codes for a protein that helps develop and maintain corneal transparency, as it is highly expressed in the cornea, as well as contributes to the structure of the stromal matrix that has a length of 352 amino acids and a weight of approximately 40.5 kDa. Studies are being conducted on severeal diseases and disorders relating to this gene, including cornea plana congenital, acrus senilis, keratopathy, corneal dystrophy, hyperopia, and glaucoma. KERA has also been shown to have interactions with CXCL2, B3GNT7, CHST1, CHST5, and CHST6 in pathways such as the MAroteaux-Lamy syndrome, metabolic, and Sanfilippo syndrome pathways.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for KERA ELISA Kit (NBP3-39698). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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