![Western Blot: KCNK12 Antibody [NBP2-81727] - Analysis of KCNK12 in rat brain tissue lysate with KCNK12 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize2/KCNK12-Antibody-Western-Blot-NBP2-81727-img0001.jpg "Western Blot: KCNK12 Antibody [NBP2-81727] - Analysis of KCNK12 in rat brain tissue lysate with KCNK12 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | KCNK12 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human KCNK12. The immunogen is located within amino acids 320 - 370 of KCNK12. Amino Acid Squence: PGSRLRRRLAALGA |
| Specificity | KCNK12 antibody is predicted to not cross-react with other KCNK protein family members. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KCNK12 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Western Blot: KCNK3 Antibody (S374-48) [NBP2-42202] - Western Blot analysis of Rat Brain Membrane showing detection of ~50 kDa TASK1 Potassium Channel protein using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (NBP2-42202). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (NBP2-42202) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~50 kDa.](http://images.novusbio.com/images2/KCNK3-Antibody-(S374-48)-Western-Blot-NBP2-42202-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK3 Antibody (S374-48) [NBP2-42202] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (NBP2-42202). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (NBP2-42202) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TASK1 Potassium Channel Antibody. (D) Composite.](http://images.novusbio.com/images2/KCNK3 Antibody (S374-48)-Immunocytochemistry-Immunofluorescence-NBP2-42202-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK3 Antibody (S374-48) [NBP2-42202] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (NBP2-42202). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (NBP2-42202) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TASK1 Potassium Channel Antibody (D) Composite.](http://images.novusbio.com/images2/KCNK3-Antibody-(S374-48)-Immunocytochemistry-Immunofluorescence-NBP2-42202-img0005.jpg)
![Immunohistochemistry: KCNK9 Antibody [NBP2-84109] - Immunohistochemistry with prostate cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-KCNK9 antibody](http://images.novusbio.com/images2/KCNK9-Antibody-Immunohistochemistry-NBP2-84109-img0001.jpg)
![Immunohistochemistry: KCNK9 Antibody [NBP2-84109] - Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-KCNK9 antibody](http://images.novusbio.com/images2/KCNK9-Antibody-Immunohistochemistry-NBP2-84109-img0002.jpg)
![Western Blot: KCNK9 Antibody [NBP2-84109] - Host: Rabbit. Target Name: KCNK9. Sample Tissue: Human Hela Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/images2/KCNK9-Antibody-Western-Blot-NBP2-84109-img0004.jpg)
![Western Blot: KCNK4 Antibody [NBP2-76938] - Western blot analysis of K2P4.1 on different lysates using anti-K2P4.1 antibody at 1/500 dilution.Positive control:Lane1: Mouse colon Lane2: Rat kidney](http://images.novusbio.com/images2/KCNK4-Antibody-Western-Blot-NBP2-76938-img0001.jpg)
![Immunohistochemistry: KCNK4 Antibody [NBP2-76938] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/KCNK4-Antibody-Immunohistochemistry-NBP2-76938-img0002.jpg)
![Immunohistochemistry: KCNK4 Antibody [NBP2-76938] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/KCNK4-Antibody-Immunohistochemistry-NBP2-76938-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK18 Antibody [NBP1-77097] - Immunofluorescence of TRESK in Rat Brain cells with TRESK antibody at 20 ug/mL.](http://images.novusbio.com/images2/KCNK18-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77097-img0003.jpg)
![Western Blot: KCNK18 Antibody [NBP1-77097] - Rat brain tissue lysate with KCNK18 antibody at (A) 1 and (B) 2 ug/mL](http://images.novusbio.com/images2/KCNK18-Antibody-Western-Blot-NBP1-77097-img0001.jpg)
![Immunohistochemistry-Paraffin: KCNK18 Antibody [NBP1-77097] - Rat brain tissue with KCNK18 antibody at 5 ug/mL.](http://images.novusbio.com/images2/KCNK18-Antibody-Immunohistochemistry-Paraffin-NBP1-77097-img0002.jpg)
![Immunohistochemistry-Paraffin: KCNK5 Antibody [NBP2-32432] - Staining of human kidney shows positivity in a subset of renal tubules.](http://images.novusbio.com/images2/KCNK5-Antibody-Immunohistochemistry-Paraffin-NBP2-32432-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK5 Antibody [NBP2-32432] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.](http://images.novusbio.com/images2/KCNK5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32432-img0008.jpg)
![Western Blot: KvBeta2 Antibody [NBP1-80271] - Antibody Titration: 1 ug/ml Human HepG2.](http://images.novusbio.com/images2/KvBeta2-Antibody-Western-Blot-NBP1-80271-img0007.jpg)
![Western Blot: KvBeta2 Antibody [NBP1-80271] - Reccomended Titration: 1.25 ug/ml Positive Control: Jurkat cell lysate](http://images.novusbio.com/images2/KvBeta2-Antibody-Western-Blot-NBP1-80271-img0008.jpg)
![Immunohistochemistry-Paraffin: KvBeta2 Antibody [NBP1-80271] - KvBeta2 Antibody Sample Type: P48 Mouse. Dilution: 1:2000.](http://images.novusbio.com/images2/KvBeta2-Antibody-Immunohistochemistry-Paraffin-NBP1-80271-img0004.jpg)
![Western Blot: KCNK1 Antibody [NBP2-76940] - Western blot analysis of KCNK1 on different cell lysate using anti-KCNK1 antibody at 1/500 dilution.Positive control:Lane1: Human placentaLane2: Human liver](http://images.novusbio.com/images2/KCNK1-Antibody-Western-Blot-NBP2-76940-img0001.jpg)
![Immunohistochemistry: KCNK1 Antibody [NBP2-76940] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/KCNK1-Antibody-Immunohistochemistry-NBP2-76940-img0002.jpg)
![Immunohistochemistry: KCNK1 Antibody [NBP2-76940] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/KCNK1-Antibody-Immunohistochemistry-NBP2-76940-img0003.jpg)
![Flow Cytometry: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Flow Cytometric Analysis of A549 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/MSH2-Antibody-(MSH2-2622)-Flow-Cytometry-NBP3-07211-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Immunofluorescence staining of MOLT-4 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (green). Membrane are stained with Phalloidin (Red).](http://images.novusbio.com/images2/MSH2-Antibody-(MSH2-2622)-Immunocytochemistry-Immunofluorescence-NBP3-07211-img0002.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Colon from Lynch disease stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/images2/MSH2-Antibody-(MSH2-2622)-Immunohistochemistry-Paraffin-NBP3-07211-img0003.jpg)
![Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HRAS antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/HRAS-Antibody-Western-Blot-NBP2-42864-img0009.jpg)
![Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/HRAS-Antibody-Western-Blot-NBP2-42864-img0011.jpg)
![Western Blot: HRAS Antibody [NBP2-42864] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:5000.](http://images.novusbio.com/images2/HRAS-Antibody-Western-Blot-NBP2-42864-img0006.jpg)
![Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.](http://images.novusbio.com/images2/CD82-Kai-1-Antibody-(C33)-Flow-Cytometry-NBP2-21792-img0001.jpg)
![Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).](http://images.novusbio.com/images2/CD82-Kai-1-Antibody-(C33)-Immunohistochemistry-Paraffin-NBP2-21792-img0002.jpg)
![Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Flow Cytometry: [PerCP] [NBP2-21792PCP] - A cell surface (A) and intracellular stain (B) was performed on Daudi cells with CD82/Kai-1 (C33) antibody NBP2-21792PCP along with a matched isotype control NB600-986PCP. The cell surface stain was performed in an antibody dilution of 5 ug/mL and incubated at RT for 20 minutes. Prior to intracellular staining, the cells were fixed with 4% PFA and then permeablized with 0.1 % saponin. The cells were then incubated in an antibody dilution of 5 ug/mL at RT for 30 minutes. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex. Image using the PerCP form of this antibody.](http://images.novusbio.com/images2/CD82-Kai-1-Antibody-(C33)-Flow-Cytometry-NBP2-21792-img0003.jpg)
![Western Blot: MCC Antibody [NBP1-85885] - Analysis using Anti-MCC antibody NBP1-85885 (A) shows similar pattern to independent antibody NBP2-48671 (B).](http://images.novusbio.com/images2/MCC-Antibody-Western-Blot-NBP1-85885-img0008.jpg)
![Western Blot: MCC Antibody [NBP1-85885] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/images2/MCC-Antibody-Western-Blot-NBP1-85885-img0007.jpg)
![Immunohistochemistry-Paraffin: MCC Antibody [NBP1-85885] - Staining of human ovary shows moderate cytoplasmic positivity in ovarian stroma cells.](http://images.novusbio.com/images2/MCC-Antibody-Immunohistochemistry-Paraffin-NBP1-85885-img0006.jpg)
![Western Blot: TBCA Antibody [NBP1-86288] - Analysis in control (vector only transfected HEK293T lysate) and TBCA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/TBCA-Antibody-Western-Blot-NBP1-86288-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TBCA Antibody [NBP1-86288] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & microtubules.](http://images.novusbio.com/images2/TBCA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86288-img0005.jpg)
![Immunohistochemistry-Paraffin: TBCA Antibody [NBP1-86288] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/TBCA-Antibody-Immunohistochemistry-Paraffin-NBP1-86288-img0002.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for KCNK12 Antibody (NBP2-81727)Discover more about diseases related to KCNK12 Antibody (NBP2-81727).
| Pathways for KCNK12 Antibody (NBP2-81727)View related products by pathway.
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PTMs for KCNK12 Antibody (NBP2-81727)Learn more about PTMs related to KCNK12 Antibody (NBP2-81727).
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| Gene Symbol | KCNK12 |
![Immunocytochemistry/Immunofluorescence: KCNK12 Antibody [NBP2-81727] - Immunofluorescence of KCNK12 in mouse brain tissue with KCNK12 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize2/KCNK12-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81727-img0003.jpg "Immunocytochemistry/Immunofluorescence: KCNK12 Antibody [NBP2-81727] - Immunofluorescence of KCNK12 in mouse brain tissue with KCNK12 antibody at 20 ug/mL.")
![Immunohistochemistry: KCNK12 Antibody [NBP2-81727] - Immunohistochemistry of KCNK12 in mouse brain tissue with KCNK12 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize2/KCNK12-Antibody-Immunohistochemistry-NBP2-81727-img0002.jpg "Immunohistochemistry: KCNK12 Antibody [NBP2-81727] - Immunohistochemistry of KCNK12 in mouse brain tissue with KCNK12 antibody at 5 ug/mL.")
![Immunohistochemistry: KCNK13 Antibody [NBP2-41132] - Human brain tissue with KCNK13 antibody at 20 ug/mL.](http://images.novusbio.com/images2/KCNK13-Antibody-Immunofluorescence-NBP2-41132-img0003.jpg)
![Immunohistochemistry-Paraffin: KCNK13 Antibody [NBP2-41132] - Human brain tissue with KCNK13 antibody at 5 ug/mL.](http://images.novusbio.com/images2/KCNK13-Antibody-Immunohistochemistry-Paraffin-NBP2-41132-img0002.jpg)
![Western Blot: KCNK13 Antibody [NBP2-41132] - Analysis of KCNK13 in rat brain tissue lysate with KCNK13 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/images2/KCNK13-Antibody-Western-Blot-NBP2-41132-img0001.jpg)
![Western Blot: TREK 1 Antibody [NB110-41535] - Detection of TREK 1 in membrane lysate fraction of human brain tissue.](http://images.novusbio.com/images2/TREK-1-Antibody-Western-Blot-NB110-41535-img0003.jpg)
![Immunohistochemistry-Paraffin: Potassium channel subfamily K member 6 Antibody [NBP2-34063] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/images2/Potassium-channel-subfamily-K-member-6-Antibody-Immunohistochemistry-Paraffin-NBP2-34063-img0001.jpg)
![ELISA: KCNK10 Antibody (1C1) [H00054207-M03] - Detection limit for recombinant GST tagged KCNK10 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/images2/KCNK10-Antibody-(1C1)-ELISA-H00054207-M03-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IFITM1 Antibody [NBP1-89345] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/IFITM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89345-img0010.jpg)
![Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining in human epididymis and testis tissues using anti-IFITM1 antibody. Corresponding IFITM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP1-89345-img0013.jpg)
![Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human epididymis shows strong membranous positivity in ependymal cells.](http://images.novusbio.com/images2/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP1-89345-img0014.jpg)
![Western Blot: FAM60A Antibody [H00058516-B02P] - Analysis of FAM60A expression in transfected 293T cell line by FAM60A polyclonal antibody. Lane 1: FAM60A transfected lysate(24.31 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/FAM60A-Antibody-Western-Blot-H00058516-B02P-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FAM60A Antibody [H00058516-B02P] - Analysis of purified antibody to FAM60A on HeLa cell. (antibody concentration 30 ug/ml)](http://images.novusbio.com/images2/FAM60A-Antibody-Immunocytochemistry-Immunofluorescence-H00058516-B02P-img0001.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")