IFITM1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, TF‑1 human erythroleukemic cell line, human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFITM1 Antigen ...read more
IFITM1 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human IFITM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4827) at 1.7 µg/mL for 3 hours at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

IFITM1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IFITM1
Met1-His36
Accession # NP_003632
Specificity
Detects human IFITM1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human (rh) IFITM2 and rhIFITM3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IFITM1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Control
IFITM1 Knockout HeLa Cell Lysate
Publications
Read Publication using
AF4827 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFITM1 Antibody [Unconjugated]

  • CD225 antigen
  • CD225
  • CD225Leu-13 antigen
  • IFI17
  • IFI17LEU13,9-27
  • IFITM1
  • interferon induced transmembrane protein 1 (9-27)
  • Interferon-induced protein 17
  • interferon-induced transmembrane protein 1
  • Interferon-inducible protein 9-27
  • Leu-13

Background

IFITM1 (Interferon inducible transmembrane protein 1; also Leu13, CD225 and "9‑27") is a 17 kDa member of the CD225 family of proteins. It is inducible in multiple cell types by both type I and II interferons, and although reported to occur on the cell surface, is apparently concentrated in the ER. Homotypic IFITM1 interactions promote p53 antiproliferative activity by blocking p53 phosphorylation on Thr55. Human IFITM1 is a 2‑transmembrane protein 125 amino acids (aa) in length. The topology of the molecule is unclear, but transmembrane segments are reported between aa 37‑57 and 87‑107. Over aa 1‑36, human IFITM1 shares only 47% aa identity with mouse IFITM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFITM1 Antibody (AF4827)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IFITM1
Uniprot