KCC2/SLC12A5 [p Thr1007] Antibody - Azide Free

Western Blot: KCC2/SLC12A5 [p Thr 1007] Antibody [NBP3-11949] - Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2/SLC12A5 protein phosphorylated at Thr1007 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).

• Reactivity: Mu, Rt, Hu, Po, Bv, Pm, Sh
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

KCC2/SLC12A5 [p Thr1007] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit KCC2/SLC12A5 [p Thr1007] Antibody - Azide Free (NBP3-11949) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr1007 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH) [UniProt Q63633].
• Modification: p Thr1007
• Specificity: Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Thr1007. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Porcine (100%), Bovine (100%), Sheep (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC12A5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for KCC2/SLC12A5 [p Thr1007] Antibody - Azide Free

• Electroneutral potassium-chloride cotransporter 2
• hKCC2
• KCC2
• KCC2K-Cl cotransporter 2
• KIAA1176erythroid K-Cl cotransporter 2
• Neuronal K-Cl cotransporter
• SLC12A5
• solute carrier family 12 (potassium/chloride transporter), member 5
• solute carrier family 12 member 5

Background

K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC12A5