KCC1/SLC12A4 Antibody

Western Blot: KCC1/SLC12A4 Antibody [NBP1-06043] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: KCC1/SLC12A4 Antibody [NBP1-06043] - (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KCC1/SLC12A4 Antibody Summary

• Immunogen: Peptide with sequence C-DPSHAPDNFRE corresponding to internal region according to NP_005063.1.
• Epitope: C-DPSHAPDNFRE
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLC12A4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.3-1ug/ml
• Application Notes: WB: Approx. 150 kDa band observed in human heart and skeletal muscle lysates (calculated MW of 121 kDa band according to NP_005063.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KCC1/SLC12A4 Antibody

• Electroneutral potassium-chloride cotransporter 1
• Erythroid K-Cl cotransporter 1
• FLJ17069
• FLJ40489
• hKCC1
• KCC1
• KCC1erythroid K:Cl cotransporter
• K-Cl cotransporter
• potassium/chloride cotransporter 1
• SLC12A4
• solute carrier family 12 (potassium/chloride transporters), member 4
• solute carrier family 12 member 4

Background

Potassium-chloride cotransporters mediate the coupled movement of potassium and chloride ions across the plasma membrane. SLC12A4 mediates electroneutral potassium-chloride cotransport when activated by cell swelling and may contribute to cell volume homeostasis in single cells. SLC12A4 may play a role in early erythroid maturation events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KCC1/SLC12A4 Antibody (NBP1-06043)(1)

Publication using NBP1-06043

• Simard CF, Bergeron MJ, Frenette-Cotton R et al. Homooligomeric and heterooligomeric associations between K+-Cl- cotransporter isoforms and between K+-Cl- and Na+-K+-Cl- cotransporters. J Biol Chem 2007-06-22 [PMID: 17462999]

Bioinformatics

• Gene Symbol: SLC12A4
• Entrez:
  • 6560(Human)
  • 20498(Mouse)
  • 29501(Rat)
• Uniprot:
  • NP_005063.1(Human)