| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59337-25ug | |||
HAF018). A specific band was detected for KCC2/SLC12A5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human brain stem tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human KCC2/SLC12A5 Monoclonal Antibody (Catalog # MAB8369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for KCC2/SLC12A5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8369-SP | |||
| Carrier Free | FAB8369V-100UG | |||
| Carrier Free | FAB8369U-100UG | |||
| Carrier Free | FAB8369T-100UG | |||
| Carrier Free | FAB8369S-100UG | |||
| Carrier Free | FAB8369R-100UG | |||
| Carrier Free | FAB8369N-100UG | |||
| Carrier Free | FAB8369G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49674-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP3-11949-25ul | |||
![Western Blot: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Western Blot analysis of Rat brain membrane lysate showing detection of KCC2/SLC12A5 protein using Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody, Clone S1-12 (NBP2-59337). Primary Antibody: Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody (NBP2-59337) at 1:1000.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-S1-12-Western-Blot-NBP2-59337-img0005.jpg "Western Blot: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Western Blot analysis of Rat brain membrane lysate showing detection of KCC2/SLC12A5 protein using Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody, Clone S1-12 (NBP2-59337). Primary Antibody: Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody (NBP2-59337) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody, Clone S1-12 (NBP2-59337). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody (NBP2-59337) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCC2/SLC12A5 Antibody (D) Composite.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-S1-12-Immunocytochemistry-Immunofluorescence-NBP2-59337-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-S1-12-Immunocytochemistry-Immunofluorescence-NBP2-59337-img0002.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0006.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13161-img0003.jpg)
![Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Immunohistochemistry-Paraffin-NBP3-13161-img0002.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0001.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0004.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP3-13161-img0005.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-91020242091487.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-9102024200103.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-91020242014613.jpg)
![Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Human brain (5 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13161_rabbit-kcc2-slc12a5-pab-910202420134099.jpg)
![Western Blot: KCC2/SLC12A5 [p Ser940] Antibody [NBP2-29513] - Rat hippocampal homogenate showing specific labeling of the ~ 135k KCC2 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/KCC2-SLC12A5-[p-Ser940]-Antibody-Western-Blot-NBP2-29513-img0002.jpg "Western Blot: KCC2/SLC12A5 [p Ser940] Antibody [NBP2-29513] - Rat hippocampal homogenate showing specific labeling of the ~ 135k KCC2 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).")
MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ![Western Blot: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-212024935557.jpg "Western Blot: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - Unboiled and boiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-2120249362734.jpg)
![Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - KCC2 antibody [HL2535] detects KCC2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. KCC2 stained by KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-2120249351378.jpg)
![Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - KCC2 antibody [HL2535] detects KCC2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. KCC2 stained by KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-2120249355574.jpg)
![Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-western-blot-1411202518435616.jpg)
![KCC2/SLC12A5 antibody [HL2535] detects KCC2/SLC12A5 protein at Glogi by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: KCC2/SLC12A5 stained by KCC2/SLC12A5 antibody [HL2535] (NBP3-25541) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25541_rabbit-kcc2-slc12a5-mab-hl2535-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518294119.jpg)
![Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP2-49674] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Immunohistochemistry-Paraffin-NBP2-49674-img0003.jpg "Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP2-49674] - Staining of human pancreas shows low expression as expected.")
![Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP2-49674] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Immunohistochemistry-Paraffin-NBP2-49674-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP2-49674] - Staining in human cerebral cortex and pancreas tissues using anti-SLC12A5 antibody. Corresponding SLC12A5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Immunohistochemistry-Paraffin-NBP2-49674-img0004.jpg)
![Western Blot: KCC2/SLC12A5 [p Thr 1007] Antibody [NBP3-11949] - Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2/SLC12A5 protein phosphorylated at Thr1007 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/KCC2-SLC12A5-[p-Thr-1007]-Antibody-Western-Blot-NBP3-11949-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: KCC2/SLC12A5 [p Thr 1007] Antibody [NBP3-11949] - Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2/SLC12A5 protein phosphorylated at Thr1007 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).")
![Western Blot: KCC2/SLC12A5 Antibody [NBP1-74063] - Mouse Brain Lysate 1ug/ml Gel Concentration 6-18%](http://images.novusbio.com/fullsize/KCC2-SLC12A5-Antibody-Western-Blot-NBP1-74063-img0002.jpg "Western Blot: KCC2/SLC12A5 Antibody [NBP1-74063] - Mouse Brain Lysate 1ug/ml Gel Concentration 6-18%")
![Western Blot: SLC12A5 Overexpression Lysate (Adult Normal) [NBL1-16006] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC12A5.](http://images.novusbio.com/fullsize/SLC12A5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16006-img0002.jpg "Western Blot: SLC12A5 Overexpression Lysate (Adult Normal) [NBL1-16006] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC12A5.")
![ELISA: Human KCC2/SLC12A5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39429] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39429_human-kcc2-slc12a5-ready-to-use-elisa-kit-colorimetric-2110202415264330.png "ELISA: Human KCC2/SLC12A5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39429] - Standard Curve Reference")
![ELISA: Human KCC2/SLC12A5 ELISA Kit (Colorimetric) [NBP3-39428] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39428_human-kcc2-slc12a5-elisa-kit-colorimetric-2110202415393760.png "ELISA: Human KCC2/SLC12A5 ELISA Kit (Colorimetric) [NBP3-39428] - Standard Curve Reference")
