KCC2/SLC12A5 [p Ser940] Antibody - Azide Free

Western Blot: KCC2/SLC12A5 [p Ser940] Antibody [NBP2-29513] - Rat hippocampal homogenate showing specific labeling of the ~ 135k KCC2 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm, Ze
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

KCC2/SLC12A5 [p Ser940] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 conjugated to KLH. Accession # Q63633
• Modification: p Ser940
• Specificity: Specific for endogenous levels of the ~135 kDa KCC2/SLC12A5 protein phosphorylated at Ser940. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.
• Predicted Species: Primate (100%), Canine (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC12A5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 40 uL of antibody per 1 mg lysate
  • Western Blot 1:1000
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Human reported in scientific literature (PMID:34500023). Mouse reactivity reported in scientific literature (PMID: 30106375).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for KCC2/SLC12A5 [p Ser940] Antibody - Azide Free

• Electroneutral potassium-chloride cotransporter 2
• hKCC2
• KCC2
• KCC2K-Cl cotransporter 2
• KIAA1176erythroid K-Cl cotransporter 2
• Neuronal K-Cl cotransporter
• SLC12A5
• solute carrier family 12 (potassium/chloride transporter), member 5
• solute carrier family 12 member 5

Background

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it's rate of internalization from the plasma membrane (Lee et al., 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KCC2/SLC12A5 Antibody (NBP2-29513)(4)

Publications using NBP2-29513

• Toritsuka M, Yoshino H, Makinodan M et al. Developmental dysregulation of excitatory-to-inhibitory GABA-polarity switch may underlie schizophrenia pathology: A monozygotic-twin discordant case analysis in human iPS cell-derived neurons Neurochemistry international 2021-09-06 [PMID: 34500023] (WB, Human)
• Barbour AJ, Nass SR, Hahn YK et al. Restoration of KCC2 Membrane Localization in Striatal Dopamine D2 Receptor-Expressing Medium Spiny Neurons Rescues Locomotor Deficits in HIV Tat-Transgenic Mice ASN Neuro 2021-08-27 [PMID: 34445881] (Western Blot, Mouse)
• Li J, Zhang L, Xu C et al. Prolonged Use of NMDAR Antagonist Develops Analgesic Tolerance in Neuropathic Pain via Nitric Oxide Reduction-Induced GABAergic Disinhibition Neurotherapeutics 2020-07-06 [PMID: 32632774]
• Dumon C, Diabira D, Chudotvorova I et al. The adipocyte hormone leptin sets the emergence of hippocampal inhibition in mice Elife 2018-08-15 [PMID: 30106375] (WB, Mouse)

Bioinformatics

• Gene Symbol: SLC12A5
• Entrez:
  • 171373(Rat)
• Uniprot:
  • Q63633(Rat)