KAT3B/p300 Antibody (RW109) - BSA Free

Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617] - p300 detected in a HeLa nuclear extract using NB100-617 (1:1,000). ECL: 20 minute exposure.

• Reactivity: Hu, Mu, Rt, Md, Pm
• Applications: WB, ChIP, ICC/IF, IP, ChIP
• Clone: RW109
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

KAT3B/p300 Antibody (RW109) - BSA Free Summary

• Immunogen: Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q09472]
• Epitope: The epitope recognized is found between amino acids 2107-2283.
• Localization: Nuclear
• Specificity: This is specific for p300 protein. This recognizes residues 2107-2283.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EP300
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: In Western Blot, a band is seen at ~300 kDa. A lower non-specific MW bands (~50 kDa) may be seen with longer exposure times.
• Theoretical MW: 300 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Nuclear Cell Lysate

Reactivity Notes

Mink.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for KAT3B/p300 Antibody (RW109) - BSA Free

• E1A binding protein p300
• E1A-associated protein p300
• E1A-binding protein, 300kD
• EC 2.3.1
• EC 2.3.1.48
• EP300
• histone acetyltransferase p300
• KAT3B
• p300 HAT
• p300
• RSTS2

Background

In eukaryotic genome, the chromatin dynamicity is crucial for regulation of processes requiring access to genetic information and it is regulated by several different factors including post-translational modifications of histone/non-histone components, especially via acetylation process which is catalyzed by enzymes known as lysine acetyltransferases (KATs). Among different KATs, KAT3B/ p300 and its homologue KAT3A/CBP (CREB-binding protein) are extensively studied KATs with well known cellular functions. KAT3B/ p300 and KAT3A/CBP have been shown to directly interact with several different DNA-binding transcription factors which includes nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Moreover, both KAT3B/p300 and KAT3A/CBP have been shown to exert HAT (histone acetyltransferase) activity, capable of acetylating all four core histone particles in nucleosomes and to play a key role in activating chromatin for transcription. KAT3B/p300 is a potent transcriptional coactivator that has been shown to regulate various biological processes such as cellular proliferation, differentiation, cell cycle, apoptosis, and response to genetic damage, and KAT3B/ p300 dysfunction interfering with these processes implicates in the pathogenesis of several diseases such as cancer, cardiac diseases, diabetes mellitus, and HIV.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KAT3B/p300 Antibody (NB100-617)(5)

Publications using NB100-617

• Tufano M, Cesaro E, Martinelli R et al. FKBP51 Affects TNF-Related Apoptosis Inducing Ligand Response in Melanoma Frontiers in Cell and Developmental Biology 2021-09-13 [PMID: 34589486]
• Romano S, Staibano S, Greco A et al. FK506 binding protein 51 positively regulates melanoma stemness and metastatic potential. Cell Death Dis. 2013-04-04 [PMID: 23559012] (WB, IP, Chemotaxis, Human)
• Eckner R et al. Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation. Genes Dev;1 (19):2478-90. 1996-10-01 [PMID: 8843199]
• Eckner R et al. Association of p300 and CBP with simian virus 40 large T antigen. Mol Cell Biol;16(7):3454-64. 1996-07-01 [PMID: 8668161] (IP, Mouse)
• Eckner R et al. Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor. Genes Dev;8(8):869-84. 1994-04-15 [PMID: 7523245] (WB, Human)

FAQs for KAT3B/p300 Antibody (NB100-617). (Showing 1 - 1 of 1 FAQs).

1. I'm in the market for a monoclonal antibody for p300/KAT3B for WB, that would react with mouse p300. In your inventory you have some listed that I saw meet these criteria. I'm interested in knowing more about the RW antibodies: RW105, RW109 and RW128. Do you know if in addition to WB, any of these can also be used in IP or ChIP?
   • Thank you for contacting Novus Biologicals regarding our p300 antibodies. The three clones you are interested in are all guaranteed to work in mouse, and in WB and IP. We haven't yet tested these in ChIP, but if you wanted to try them and let us know how those results turn out you actually qualify for our Innovators Reward Program.

Bioinformatics

• Gene Symbol: EP300
• Entrez:
  • 2033(Human)
  • 328572(Mouse)
  • 170915(Rat)