KAP1 Antibody (20C1)

Western Blot: KAP1 Antibody (20C1) [NB100-74549] - Analysis of TIF1 beta in gamma irradiated HeLA cells.

Immunocytochemistry/Immunofluorescence: KAP1 Antibody (20C1) [NB100-74549] - Gamma irradiated HeLa cells.

• Reactivity: Hu, Mu
• Applications: WB, GS, ICC/IF, IHC, IP, ChIP
• Clone: 20C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

KAP1 Antibody (20C1) Summary

• Description: Novus Biologicals Mouse KAP1 Antibody (20C1) (NB100-74549) is a monoclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-KAP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein.
• Specificity: TIF1 beta (20C1)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM28
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/ Immunofluorescence 2 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/ml

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22110054). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for KAP1 Antibody (20C1)

• E3 SUMO-protein ligase TRIM28
• EC 6.3.2.-
• FLJ29029
• KAP1
• KAP-1
• KAP1KRAB-associated protein 1
• KRIP-1
• Nuclear corepressor KAP-1
• RNF96
• RNF96KRAB-interacting protein 1
• TF1B
• TIF1B
• TIF1-beta
• TIF1BRING finger protein 96
• transcription intermediary factor 1-beta
• transcriptional intermediary factor 1-beta
• TRIM28
• tripartite motif containing 28
• tripartite motif-containing 28
• Tripartite motif-containing protein 28

Background

TIF1 beta is a member of the tripartite motif (TRIM) family. It is composed of a N-terminal RBCC (RING finger, B boxes, coiled coil) motif, and a C-terminal bromodomain which is preceded by a PHD finger. TIF1 beta has been reported to function as a co-repressor for Kruppel-associated box (KRAB)-domain-containing zinc finger proteins. As such, it plays a crucial role in early embryogenesis. It appears that the co-repressor function of TIF1 beta is related to its organization and/or maintenance of higher-order, heterochromatin-like structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KAP1 Antibody (NB100-74549)(1)

Publication using NB100-74549

• Jasmine Manji, Jasmine Pipella, Gabriel Brawerman, Peter J. Thompson, Assam El-Osta Exploring Transcriptional Regulation of Beta Cell SASP by Brd4-Associated Proteins and Cell Cycle Control Protein p21 Epigenomes 2024-03-06 [PMID: 38534794]

Bioinformatics

• Gene Symbol: TRIM28
• Entrez:
  • 10155(Human)
  • 21849(Mouse)