Kallikrein 4/Prostase/EMSP1 Antibody (325712)

Kallikrein 4/Prostase/EMSP1 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Kallikrein 4/Prostase/EMSP1 Monoclonal Antibody (Catalog # MAB35661) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP, B/N
• Clone: 325712
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Kallikrein 4/Prostase/EMSP1 Antibody (325712) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 4/Prostase/EMSP1
  Ser27-Ser254
  Accession # NP_004908
• Specificity: Detects human Kallikrein 4/Prostase/EMSP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Kallikrein 1, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, rhKallikrein B1, rhFactor VII, rhFactor X, rhFactor XI, rhHGFA, rhThrombin, and rhUPA-1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLK4
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 2.9 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 4/Prostase/EMSP1 Antibody (325712)

• AI2A1
• ARM1
• EC 3.4.21
• EC 3.4.21.-
• EMSP
• EMSP1
• EMSP1MGC116827
• Enamel matrix serine proteinase 1
• kallikrein 4 (prostase, enamel matrix, prostate)
• Kallikrein 4
• kallikrein-4
• Kallikrein-like protein 1
• kallikrein-related peptidase 4
• KLK4
• KLK-L1MGC116828
• Prostase
• PRSS17enamel matrix serine protease 1
• PSTSandrogen-regulated message 1
• Serine protease 17

Background

Kallikrein 4 (KLK4), also known as prostase or EMSP1 (enamel matrix serine protease 1), is a serine protease of the human tissue kallikrein gene family (1). Among normal tissues, human KLK4 is specifically expressed in the prostate (2). It is over-expressed in prostate cancer and this expression is regulated by hormones including androgens, esterogen and progesterone (3). Recombinant human KLK4 readily activates pro-KLK3/PSA and pro-urokinase type plasminogen activator (uPA), indicating it may initiate events involving PSA and uPA in either normal or abnormal processes (4). KLK4 may have additional roles such as functioning as one of the two major enamel proteases identified that process enamel matrix proteins (5). In addition to being a secreted enzyme, it is also a nuclear protein (3, 6). The deduced amino acid sequence of human KLK4 consists of a signal peptide, a short pro region and a mature/active enzyme.

1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
2. Nelson, P.S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3114.
3. Xi, Z. et al. (2004) Cancer Res. 64:2365.
4. Takayama, T.K. et al. (2001) Biochemistry 40:15341.
5. Simmer, J.P. and J.C. Hu (2002) Connect Tissue Res. 43:441.
6. Ryu, O.H. et al. (2002) Eur. J. Oral. Sci. 110:358.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLK4
• Uniprot:
  • NP_004908()