MMP-25/MT6-MMP Antibody (141808) [Unconjugated]

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 141808
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MMP-25/MT6-MMP Antibody (141808) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP‑25/MT6-MMP
  Pro314-Cys508
  Accession # Q9NPA2
• Specificity: Detects human MMP‑25/MT6-MMP in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP25
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-25/MT6-MMP Antibody (141808) [Unconjugated]

• Leukolysin
• matrix metallopeptidase 25
• matrix metallopeptidase-like 1
• matrix metalloproteinase 20
• matrix metalloproteinase 25
• matrix metalloproteinase-25
• matrix metalloproteinase-like 1
• membrane-type 6 matrix metalloproteinase
• membrane-type matrix metalloproteinase 6
• membrane-type-6 matrix metalloproteinase
• MMP20
• MMP20A
• MMP25
• MMP-25
• MMPL1
• MT6MMP
• MT6-MMP
• MT-MMP 6
• MTMMP6
• MT-MMP6

Background

MMP-25, also known as MT6-MMP or Leukolysin, is a GPI-linked protease expressed by neutrophils. MMP-25 cleaves collagen type 4, gelatin, fibronectin and fibrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-25/MT6-MMP Antibody (MAB1142)(2)

Publications using MAB1142

• Zhao H, Sohail A, Sun Q, Shi Q, Kim S, Mobashery S, Fridman R Identification and role of the homodimerization interface of the glycosylphosphatidylinositol-anchored membrane type 6 matrix metalloproteinase (MMP25). J. Biol. Chem., 2008;283(50):35023-32. 2008 [PMID: 18936094] (Western Blot, Human)
• Sun Q, Weber CR, Sohail A, Bernardo MM, Toth M, Zhao H, Turner JR, Fridman R MMP25 (MT6-MMP) is highly expressed in human colon cancer, promotes tumor growth, and exhibits unique biochemical properties. J. Biol. Chem., 2007;282(30):21998-2010. 2007 [PMID: 17513868] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MMP25
• Uniprot:
  • Q9NPA2()