Kallikrein 3/PSA Antibody [Unconjugated]

1344-SE), DU145 human prostate carcinoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 3/PSA at approximately 28-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > 1344-SE), DU145 human prostate carcinoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 3/PSA at approximately 28-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of Recombinant Human Kallikrein 3/PSA (Catalog # 1344-SE), DU145 human prostate carcinoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 3/PSA at approximately 28-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of Recombinant Human Kallikrein 3/PSA (Catalog # <a class=

HAF019). A specific band was detected for Kallikrein 3/PSA at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF019). A specific band was detected for Kallikrein 3/PSA at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Kallikrein 3/PSA at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of LNCaP cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of LNCaP cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line (positive control, left panel) and MCF-7 human breast cancer cell line (negative control, right panel) using Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of LNCaP cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line (positive control, left panel) and MCF-7 human breast cancer cell line (negative control, right panel) using Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." > CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." title="Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." />

Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Simple Western lane view shows lysates of human prostate cancer, loaded at 0.2 mg/ml. A specific band was detected for Kallikrein 3/PSA at approximately 40 kDa (as indicated) using 5 µg/ml of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 043-522-2). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

1344-SE) was serially diluted and captured by Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13442) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 1344-SE) was serially diluted and captured by Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13442) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human Kallikrein 3/PSA (Catalog # 1344-SE) was serially diluted and captured by Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13442) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human Kallikrein 3/PSA (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, IP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Kallikrein 3/PSA Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 3
  Ala18-Pro261
  Accession # P07288
• Specificity: Detects human KLK3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLK3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5-1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 3/PSA Antibody [Unconjugated]

• APS seminin
• EC 3.4.21
• EC 3.4.21.77
• gamma-seminoprotein
• hK3
• Kallikrein 3
• kallikrein 3, (prostate specific antigen)
• Kallikrein-3
• kallikrein-related peptidase 3
• KLK2A1
• KLK3
• P-30 antigen
• prostate specific antigen
• prostate-specific antigen
• PSA semenogelase
• PSA
• Semenogelase
• Seminin

Background

Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family (1). PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5 - 2 g/L) (2). A small portion of PSA “leaks” into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue (3). PSA has become a well established tumor marker that aids the diagnosis, staging, and follow up of prostate cancer.

The deduced amino acid sequence of human PSA consists of a signal peptide, a short pro region and a mature/active enzyme. The pro-enzyme is activated, possibly by active Kallikreins 2, 4 or 15 in vivo (4). rhPSA is activated by thermolysin, a zinc protease. The active PSA cleaves several tyrosyl peptide bonds in semenogelins I and II, which are the major gel-forming proteins produced by the seminal vesicles (5). Several inhibitors including serpin A3/ alpha ₁-antichymotrypsin (ACT) and alpha ₂-macroglobulin are known to form complexes with PSA.

1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
2. Ward, A.M. et al. (2001) Ann. Clin. Biochem. 38:633.
3. Jain, S. et al. (2002) Postgrad. Med. J. 78:646.
4. LiLja H. (2003) Urology 62:27.
5. Takayama, T.K. et al. (1997) J. Biol. Chem. 272:21582.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 3/PSA Antibody (AF1344)(9)

Publications using AF1344

• Jin, Y;Huang, Z;Xu, B;Chen, J; Localization of multiple DNAzymes as a branchedzyme-powered nanodevice for the immunoassay of tumor biomarkers Analytica chimica acta 2023-09-15 [PMID: 37455088] (ELISA Capture, Human, N/A)
• A Hlavá?ek, Z Farka, MJ Mickert, U Kostiv, JC Brandmeier, D Horák, P Skládal, F Foret, HH Gorris Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging Nature Protocols, 2022-02-18;0(0):. 2022-02-18 [PMID: 35181766] (Immunoprecipitation, Human)
• Adheesha N. Danthanarayana, Erin Finley, Binh Vu, Katerina Kourentzi, Richard C. Willson, Jakoah Brgoch A multicolor multiplex lateral flow assay for high-sensitivity analyte detection using persistent luminescent nanophosphors† Analytical Methods 6/25/2020 [PMID: 32577135]
• Adheesha N. Danthanarayana, Erin Finley, Binh Vu, Katerina Kourentzi, Richard C. Willson, Jakoah Brgoch A multicolor multiplex lateral flow assay for high-sensitivity analyte detection using persistent luminescent nanophosphors† Analytical Methods 2020-06-25 [PMID: 32577135] (Bioassay, N/A)
• L Chen, RS Hsi, F Yang, BA Sherer, ML Stoller, SP Ho Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex PLoS ONE, 2017-11-16;12(11):e0187103. 2017-11-16 [PMID: 29145401] (IHC-Fr, Human)
• Sarah A. Loddick, Sarah J. Ross, Andrew G. Thomason, David M. Robinson, Graeme E. Walker, Tom P.J. Dunkley et al. AZD3514: A Small Molecule That Modulates Androgen Receptor Signaling and Function In Vitro and In Vivo Molecular Cancer Therapeutics 9/1/2013 [PMID: 23861347]
• Sarah A. Loddick, Sarah J. Ross, Andrew G. Thomason, David M. Robinson, Graeme E. Walker, Tom P.J. Dunkley et al. AZD3514: A Small Molecule That Modulates Androgen Receptor Signaling and Function In Vitro and In Vivo Molecular Cancer Therapeutics 2013-09-01 [PMID: 23861347] (Western Blot, Human)
• Nora Theilacker, Eric E. Roller, Kristopher D. Barbee, Matthias Franzreb, Xiaohua Huang Multiplexed protein analysis using encoded antibody-conjugated microbeads Journal of The Royal Society Interface 8/7/2011 [PMID: 21247950]
• Klyushnenkova EN, Kouiavskaia DV, Berard CA, Alexander RB Cutting edge: Permissive MHC class II allele changes the pattern of antitumor immune response resulting in failure of tumor rejection. J. Immunol., 2009-02-01;182(3):1242-6. 2009-02-01 [PMID: 19155468] (IHC-P, Human)

Bioinformatics

• Gene Symbol: KLK3
• Uniprot:
  • P07288()