Kallikrein 3/PSA Antibody [Unconjugated]

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Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated ...read more
Western blot shows lysates of Recombinant Human Kallikrein 3/PSA (Catalog # 1344-SE), DU145 human prostate carcinoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL ...read more
Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line (positive control, left panel) and MCF‑7 human breast cancer cell line (negative control, right panel) using Goat ...read more
Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) at 15 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Kallikrein 3/PSA Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 3
Ala18-Pro261
Accession # P07288
Specificity
Detects human KLK3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) KLK2 is observed and less than 5% cross-reactivity with rhKLK1, rhKLK4, rhKLK5, rhKLK7, rhKLK8, rhKLK9, rhKLK10, rhKLK11, rhKLK12, rhKLK13, and rhKLK15 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KLK3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF1344 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 3/PSA Antibody [Unconjugated]

  • APS seminin
  • EC 3.4.21
  • EC 3.4.21.77
  • Gamma-seminoprotein
  • hK3
  • Kallikrein 3
  • kallikrein 3, (prostate specific antigen)
  • Kallikrein-3
  • kallikrein-related peptidase 3
  • KLK2A1
  • KLK3
  • P-30 antigen
  • prostate specific antigen
  • prostate-specific antigen
  • PSA semenogelase
  • PSA
  • Semenogelase
  • Seminin

Background

Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family (1). PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5 - 2 g/L) (2). A small portion of PSA “leaks” into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue (3). PSA has become a well established tumor marker that aids the diagnosis, staging, and follow up of prostate cancer.

The deduced amino acid sequence of human PSA consists of a signal peptide, a short pro region and a mature/active enzyme. The pro-enzyme is activated, possibly by active Kallikreins 2, 4 or 15 in vivo (4). rhPSA is activated by thermolysin, a zinc protease. The active PSA cleaves several tyrosyl peptide bonds in semenogelins I and II, which are the major gel-forming proteins produced by the seminal vesicles (5). Several inhibitors including serpin A3/ alpha 1-antichymotrypsin (ACT) and alpha 2-macroglobulin are known to form complexes with PSA.

  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Ward, A.M. et al. (2001) Ann. Clin. Biochem. 38:633.
  3. Jain, S. et al. (2002) Postgrad. Med. J. 78:646.
  4. LiLja H. (2003) Urology 62:27.
  5. Takayama, T.K. et al. (1997) J. Biol. Chem. 272:21582.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Kallikrein 3/PSA Antibody (AF1344)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-Fr, IHC-P.


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(1)
IHC-P
(1)
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Bioinformatics

Gene Symbol KLK3
Uniprot