Kallikrein 3/PSA Antibody (5A11E9)

Images

 
Western Blot: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis against truncated Prostate Specific Antigen recombinant protein.
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis of paraffin-embedded human prostate carcinoma tissues, showing cytoplasmic localization using Prostate Specific Antigen mouse mAb ...read more
Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Flow cytometric analysis of PC-3 cells using Prostate Specific Antigen mouse mAb (right) and negative control (left).
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis of paraffin-embedded human prostate tissues using Prostate Specific Antigen mouse mAb with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clone
5A11E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Kallikrein 3/PSA Antibody (5A11E9) Summary

Immunogen
Purified recombinant fragment of Prostate Specific Antigen (amino acids 26-251) expressed in E. coli. [UniProt# P07288]
Localization
Secreted.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KLK3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This Prostate Specific Antigen (5A11E9) antibody is useful for Western blot, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and ELISA.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified

Alternate Names for Kallikrein 3/PSA Antibody (5A11E9)

  • APS seminin
  • EC 3.4.21
  • EC 3.4.21.77
  • Gamma-seminoprotein
  • hK3
  • Kallikrein 3
  • kallikrein 3, (prostate specific antigen)
  • Kallikrein-3
  • kallikrein-related peptidase 3
  • KLK2A1
  • KLK3
  • P-30 antigen
  • prostate specific antigen
  • prostate-specific antigen
  • PSA semenogelase
  • PSA
  • Semenogelase
  • Seminin

Background

Prostate Specific Antigen (PSA, Kallikrein-3, KLK3) is a serine protease that is usually secreted from the prostate gland and presented in seminal plasma. Prostate Specific Antigen functions in the liquefaction of seminal coagulum, through hydrolysis of the high molecular mass seminal vesicle protein. Its substrates includes semenogelin I & II, fibronectin, insulin-like growth factor binding protein 3 (IGBP3), and laminin. Highly expressed by normal and malignant prostate cancer cells, Prostate Specific Antigen has been implicated in the growth, invasion, and metastasis of prostate cancer cells. Therefore, Prostate Specific Antigen is widely used as a diagnostic marker for prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Kallikrein 3/PSA Antibody (NBP1-51541). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for a pair of antibodies against TOTAL PSA to be used in a sandwich immunoassay. I saw that you carry a biotiny-Ab (NBP1-44951). I was wondering if it can be used to detect TOTAL PSA and if you recommend a matched pair?
    • A list of our PSA antibodies suitable for use in ELISA can be found using this link. None have been paired specifically with NBP1-44951 but any monoclonal on this list should suffice. I would recommend NBP1-51541 as it is our best selling antibody to this target and should be compatible with NBP1-44951.

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Bioinformatics

Gene Symbol KLK3
Entrez
Uniprot