Kallikrein 1 Antibody

Western blot shows lysates of mouse pancreas tissue and mouse small intestine. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse Kallikrein 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7928) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kallikrein 1 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Kallikrein 1 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Kallikrein 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7928) at 1.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

Kallikrein 1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein 5 precursor/Kallikrein 1
  Val23-Arg67(Kallikrein 5 precursor), Ile25-Asp261(Kallikrein 1)
  Accession # NP_081082, P15947
• Specificity: Detects mouse Kallikrein 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Kallikrein 1, recombinant mouse (rm) Kallikrein 5 and rmKallikrein 7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KLK1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 1 Antibody

• EC 3.4.21
• EC 3.4.21.35
• glandular kallikrein 1
• hK1
• Kallikrein 1
• kallikrein 1, renal/pancreas/salivary
• kallikrein serine protease 1
• kallikrein-1
• Kidney/pancreas/salivary gland kallikrein
• KLK1
• Klk6
• KLKR
• Tissue kallikrein

Background

The kallikreins are a family of trypsin‑like serine proteases, many of which are associated with a variety of cancers. Kallikrein 1 (KLK1) also known as tissue kallikrein and urinary kallikrein is a 25 - 30 kDa member of peptidase S1 family of proteins. At least two isoforms are predicted. An important physiological function of KLK1 is the cleavage of kininogen to release a vasoactive kinin peptide, bradykinin (for rodent KLK1) or lysyl‑bradykinin (for human KLK1). Kinins regulate vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation.  Over aa 25-261, mouse KLK1 shares 64% aa identity with human KLK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLK1
• Uniprot:
  • NP_081082, P15947()