Western blot shows lysates of human skin tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) ...read more
Kallikrein 7 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) overnight at 4 °C. ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Kallikrein 7 Antibody [Unconjugated]
kallikrein 7 (chymotryptic, stratum corneum)
kallikrein-related peptidase 7
protease, serine, 6
Serine protease 6
Stratum corneum chymotryptic enzyme
Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length hK7 consists of 253 amino acids, with a signal peptide (residues 1-22), short pro peptide (residues 23-29) and mature chain (residues 30-252) (1). Predominantly expressed in the skin, a major physiological function of hK7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for hK7 is hK5, another member of the human tissue Kallikrein family. Along with hK14, these three kallikreins form a proteolytic cascade in the stratum corneum (5). The purified, secreted rhK7 corresponds to the pro form. When activated by thermolysin, it displays enzymatic activity towards a fluorogenic synthetic peptide described in the Activity Assay Protocol. This activity can be inhibited by rhSerpin A1, A3, A4, and A5 (R&D Systems, Catalog # 1268‑PI, 1295-PI, 1669-PI, and 1266-PI).
Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Kallikrein 7 Antibody [Unconjugated] and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.