Kallikrein 7 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human skin tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) ...read more
Kallikrein 7 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) overnight at 4 °C. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Kallikrein 7 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 7
Glu23-His252
Accession # P49862
Specificity
Detects human and mouse Kallikrein 7 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KLK7
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publications using
AF2624 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 7 Antibody [Unconjugated]

  • EC 3.4.21
  • EC 3.4.21.117
  • hK7
  • hSCCE
  • kallikrein 7 (chymotryptic, stratum corneum)
  • Kallikrein 7
  • kallikrein-related peptidase 7
  • KLK7
  • protease, serine, 6
  • PRSS6
  • SCCEkallikrein-7
  • Serine protease 6
  • signal protein
  • Stratum corneum chymotryptic enzyme

Background

Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length hK7 consists of 253 amino acids, with a signal peptide (residues 1-22), short pro peptide (residues 23-29) and mature chain (residues 30-252) (1). Predominantly expressed in the skin, a major physiological function of hK7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for hK7 is hK5, another member of the human tissue Kallikrein family. Along with hK14, these three kallikreins form a proteolytic cascade in the stratum corneum (5). The purified, secreted rhK7 corresponds to the pro form. When activated by thermolysin, it displays enzymatic activity towards a fluorogenic synthetic peptide described in the Activity Assay Protocol. This activity can be inhibited by rhSerpin A1, A3, A4, and A5 (R&D Systems, Catalog # 1268‑PI, 1295-PI, 1669-PI, and 1266-PI).
  1. Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
  2. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
  3. Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
  4. Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
  5. Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Kallikrein 7 Antibody (AF2624)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, Western Blot.


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IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
Western Blot
(2)
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Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF2624 Applications Species
H Ma, A Hockla, C Mehner, M Coban, N Papo, DC Radisky, ES Radisky PRSS3/Mesotrypsin and kallikrein-related peptidase 5 are associated with poor prognosis and contribute to tumor cell invasion and growth in lung adenocarcinoma Sci Rep, 2019;9(1):1844. 2019 [PMID: 30755669] (Western Blot, Human) Western Blot Human
K Kidana, T Tatebe, K Ito, N Hara, A Kakita, T Saito, S Takatori, Y Ouchi, T Ikeuchi, M Makino, TC Saido, M Akishita, T Iwatsubo, Y Hori, T Tomita Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice EMBO Mol Med, 2018;0(0):. 2018 [PMID: 29311134] (IHC-Fr, Mouse) IHC-Fr Mouse
Dorn, Julia, Gkazepis, Apostolo, Kotzsch, Matthias, Kremer, Marcus, Propping, Corinna, Mayer, Katharin, Mengele, Karin, Diamandis, Elefther, Kiechle, Marion, Magdolen, Viktor, Schmitt, Manfred Clinical value of protein expression of kallikrein-related peptidase 7 (KLK7) in ovarian cancer. Biol Chem, 2014;395(1):95-107. 2014 [PMID: 23999494] (IHC, Human) IHC Human
Munoz-Repeto I, Garcia M, Kamieniak M, Ramon Y Cajal T, Domingo S, Cazorla A, Garcia Donas J, Hernando Polo S, Garcia Sagredo J, Hernandez E, Lacambra C, Saez R, Robles L, Borrego S, Prat J, Palacios J, Benitez J Phenotypic characterization of hereditary epithelial ovarian cancer based on a tissue microarray study. Histol Histopathol, 2013;28(1):133-44. 2013 [PMID: 23233066] (IHC-P, Human) IHC-P Human
Ramani VC, Haun RS The extracellular matrix protein fibronectin is a substrate for kallikrein 7. Biochem. Biophys. Res. Commun., 2008;369(4):1169-73. 2008 [PMID: 18343220] (Western Blot, Human) Western Blot Human
Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma. Biol. Chem., 2007;388(11):1235-42. 2007 [PMID: 17976017] (IHC-P, Human) IHC-P Human

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Secondary Antibodies

 

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Biotin BAF2624

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Bioinformatics

Gene Symbol KLK7
Uniprot