Recombinant Human Kallikrein 5 Protein, CF

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Recombinant Human Kallikrein 5 (Catalog # 1108-SE) is measured byits ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human Kallikrein 5 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011). The specific activity is >200 pmol/min/µg, as measured under the described conditions. 
Source
Mouse myeloma cell line, NS0-derived human Kallikrein 5 protein
Ile67-Ser293, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ile67
Structure / Form
Mature form
Protein/Peptide Type
Recombinant Enzymes
Gene
KLK5
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
37-42 kDa, reducing conditions
Publications
Read Publications using
1108-SE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Assay Procedure
  • Assay Buffer: 0.1 M NaH2PO4, pH 8.0
  • Recombinant Human Kallikrein 5 (rhKLK5) (Catalog # 1108-SE)
  • Fluorogenic Peptide Substrate BOC-Val-Pro-Arg-AMC (Catalog # ES011), 10 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhKLK5 to 2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 200 µM in Assay Buffer.
  3. Load 50 µL of the 2 ng/µL rhKLK5 into plate and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 200 µM Substrate without any rhKLK5.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC) (Sigma, Catalog # A9891)

Per Well:
  • rhKLK5: 0.1 µg
  • Substrate: 100 µM


























Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Kallikrein 5 Protein, CF

  • EC 3.4.21
  • EC 3.4.21.4
  • Kallikrein 5
  • Kallikrein-like protein 2
  • kallikrein-related peptidase 5
  • KLK5
  • KLKL2
  • KLK-L2
  • KLK-L2EC 3.4.21.-
  • SCTE
  • SCTEkallikrein-5
  • Stratum corneum tryptic enzyme

Background

Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (2). The deduced amino acid sequence of human KLK5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). KLK5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). KLK5 overexpression is an indicator of poor prognosis in ovarian cancer (4). hK5 is a candidate physiological activator of hK7 in the stratum corneum (5). Indeed, rhK5 can be used to activate rhK7 (R&D Systems, Catalog # 2624‑SE) by incubating rhK5 with recombinant human (rh) K7 (both at 0.1 mg/mL) in 25 mM MES, 0.15 M NaCl, pH 5.5, at 37 °C overnight.

rhK5 enzymatic activity can be inhibited by rhSerpinA1, A5, F2, and G1 (R&D Systems, Catalog # 1268‑PI, 1266‑PI, 1470-PI, 2488‑PI).

  1. Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
  2. Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.
  3. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  4. Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.
  5. Kim, H. et al. (2001) Br. J. Cancer 84:643.

Publications for Kallikrein 5 (1108-SE)(19)

We have publications tested in 6 confirmed species: Human, Mouse, Plant - Betula alba, Plant - Empetrum nigrum, Virus, Virus - Influenza A.

We have publications tested in 5 applications: Bioassay, ELISA (Standard), Enzyme Assay, In Vivo, Surface Plasmon Resonance.


Filter By Application
Bioassay
(11)
ELISA (Standard)
(1)
Enzyme Assay
(5)
In Vivo
(2)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(11)
Mouse
(2)
Plant - Betula alba
(1)
Plant - Empetrum nigrum
(1)
Virus
(1)
Virus - Influenza A
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using 1108-SE Applications Species
S Smiljanic, C Messaraa, V Lafon-Kolb, N Hrapovic, N Amini, C Osterlund, L Visdal-Joh Betula alba Bark Extract and Empetrum nigrum Fruit Juice, a Natural Alternative to Niacinamide for Skin Barrier Benefits International Journal of Molecular Sciences, 2022-10-19;23(20):. 2022-10-19 [PMID: 36293365] (Bioassay, Plant - Betula alba, Plant - Empetrum nigrum) Bioassay Plant - Betula alba, Plant - Empetrum nigrum
KB Roh, Y Jang, E Cho, D Park, DH Kweon, E Jung Chlorogenic Acid Isomers Isolated from Artemisia lavandulaefolia Exhibit Anti-Rosacea Effects In Vitro Biomedicines, 2022-02-16;10(2):. 2022-02-16 [PMID: 35203672] (Bioassay, Human) Bioassay Human
T Nargis, K Kumar, AR Ghosh, A Sharma, D Rudra, D Sen, S Chakrabart, S Mukhopadhy, D Ganguly, P Chakrabart KLK5 induces shedding of DPP4 from circulatory Th17 cells in type 2 diabetes Mol Metab, 2017-09-27;6(11):1529-1539. 2017-09-27 [PMID: 29107298] (Bioassay, Human) Bioassay Human
Y Matsubara, T Matsumoto, J Koseki, A Kaneko, S Aiba, K Yamasaki Inhibition of Human Kallikrein 5 Protease by Triterpenoids from Natural Sources Molecules, 2017-10-27;22(11):. 2017-10-27 [PMID: 29077044] (Bioassay, Human) Bioassay Human
M Magnen, F Gueugnon, A Guillon, T Baranek, VC Thibault, A Petit-Cour, SJ de Veer, J Harris, AA Humbles, M Si-Tahar, Y Courty Kallikrein-related peptidase 5 contributes to H3N2 influenza virus infection in human lungs J. Virol., 2017-07-27;0(0):. 2017-07-27 [PMID: 28615200] (Enzyme Assay, Virus) Enzyme Assay Virus
N Fortelny, GS Butler, CM Overall, P Pavlidis Protease-inhibitor interaction predictions: Lessons on the complexity of protein-protein interactions Mol. Cell Proteomics, 2017-04-06;0(0):. 2017-04-06 [PMID: 28385878] (Bioassay) Bioassay
MR Straus, GR Whittaker A peptide-based approach to evaluate the adaptability of influenza A virus to humans based on its hemagglutinin proteolytic cleavage site PLoS ONE, 2017-03-30;12(3):e0174827. 2017-03-30 [PMID: 28358853] (Enzyme Assay, Virus - Influenza A) Enzyme Assay Virus - Influenza A
Nathalie Castex-Riz Anti-inflammatory and immunomodulatory effects of Aquaphilus dolomiae extract on in vitro models Clin Cosmet Investig Dermatol, 2016-11-10;9(0):421-434. 2016-11-10 [PMID: 27877060] (Bioassay, Human) Bioassay Human
Defects in Stratum Corneum Desquamation Are the Predominant Effect of Impaired ABCA12 Function in a Novel Mouse Model of Harlequin Ichthyosis PLoS ONE, 2016-08-23;11(8):e0161465. 2016-08-23 [PMID: 27551807] (Bioassay, In Vivo, Human, Mouse) Bioassay, In Vivo Human, Mouse
Yamamoto-Tanaka M, Motoyama A, Miyai M, Matsunaga Y, Matsuda J, Tsuboi R, Hibino T Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation. J Biol Chem, 2014-05-28;289(29):20026-38. 2014-05-28 [PMID: 24872419] (Bioassay, Human) Bioassay Human
Show All 19 Publications.

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Bioinformatics

Gene Symbol KLK5
Uniprot