KA1/GRIK4/Glutamate Receptor KA1 Antibody (2915C) [Unconjugated]

KA1/GRIK4 was detected in immersion fixed paraffin-embedded sections of Human Brain Cortex using Rabbit Anti-Human KA1/GRIK4 Monoclonal Antibody (Catalog # MAB113261) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glial cells and in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 2915C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

KA1/GRIK4/Glutamate Receptor KA1 Antibody (2915C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human KA1/GRIK containing peptide
  Accession # P42263
• Specificity: Detects human KA1/GRIK in direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KA1/GRIK4/Glutamate Receptor KA1 Antibody (2915C) [Unconjugated]

• EAA1
• Excitatory amino acid receptor 1
• GluK4
• glutamate receptor KA1
• Glutamate receptor KA-1
• glutamate receptor, ionotropic kainate 4
• glutamate receptor, ionotropic, kainate 4
• GRIK4
• KA1
• KA1GRIK

Background

GRIK4 (Glutamate receptor, ionotropic kainate 4) is a receptor for glutamate and is one of the 5 types of kainate receptor subunits.  Glutamate acts as an excitatory neurotransmitter at many synapses in the CNS are key mediators of brain communication.  Kainate receptors are relevant targets for neuroprotection of both neurons and glia in acute and chronic neurodegerative diseases.  They are also involved in epilepsy, pain and psychiatric disorders.  Variations in GRIK4 have been associated with both increased and decreased risk of bipolar disorder.  Single-nucleotide polymorphism's in GRIK 4 are also observed in Schizophrenia.

1. Entrez Gene: GRIK4 glutamate receptor, ionotropic, kainate 4.
2. Szpirer C, Molne M, Antonacci R, Jenkins NA, Finelli P, Szpirer J, Riviere M, Gilbert DJ, Copeland NG, et al.  "The Genes Encoding the Glutamate Receptor Subunits Ka1 and KA2 (GRIK4 and GRIK5) are located on separate chromosomes in Human, Mouse and Rat". Proc Natl Acad Sci U.S.A. 1995 Jan; 91(25):11849-53.
3. Contractor A, Mulle C, Swanson GT. "Kainate Receptors Coming of Age: Milestones of Two Decades of Research". Trends in Neurosciences. 2011 Mar; 34(3):154.
4. Matute C. "Therapeutic Potential of Kainate Receptors". CNS Neuroscience & Therapeutics. 2011 Dec; 17(6):661.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P42263(Human)