JunD [p Ser255] Antibody (SR1628) - BSA Free

Western Blot: JunD [p Ser 255] Antibody (SR1628) [NBP3-21564] - Western blot analysis using NBP3-21564 in (1) HeLa cell lysate treated with Alkaline Phosphatase; (2) HeLa cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clone: SR1628
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

JunD [p Ser255] Antibody (SR1628) - BSA Free Summary

• Description: Novus Biologicals Rabbit JunD [p Ser255] Antibody (SR1628) - BSA Free (NBP3-21564) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-JunD [Ser255] (Uniprot #: P17535)
• Modification: p Ser255
• Specificity: Detects endogenous levels of phospho-JunD [Ser255]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: JUND
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for JunD [p Ser255] Antibody (SR1628) - BSA Free

• activator protein 1
• AP-1
• jun D proto-oncogene
• JunD
• JunD-FL isoform
• transcription factor jun-D

Background

The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JUND