FRA-1/FOSL1 Antibody (OTI12F9)

Western Blot: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.

Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Carcinoma of bladder tissue.

Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FOSL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FOSL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOSL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded colon tissue using anti-FOSL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded endometrium tissue using anti-FOSL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Kidney tissue using anti-FOSL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded prostate tissue using anti-FOSL1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P
• Clone: OTI12F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.1 mg/ml

FRA-1/FOSL1 Antibody (OTI12F9) Summary

• Immunogen: Full-length protein expressed in 293T cell transfected with human FOSL1 expression vector
• Specificity: This antibody is specific for Homo sapiens FOS-like antigen 1 (FOSL1).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOSL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 29.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FRA-1/FOSL1 Antibody (OTI12F9)

• FOSL1
• FOS-like antigen 1
• FOS-like antigen-1
• fos-related antigen 1
• FRA
• FRA1
• FRA-1

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FRA-1/FOSL1 Antibody (NBP1-47757)(1)

Publication using NBP1-47757

• Zolotarenko A, Chekalin E, Piruzian E, Bruskin S. FRA1 mediates the activation of keratinocytes: Implications for the development of psoriatic plaques. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2018-09-01 [PMID: 30318470] (IHC-P, Human)

Bioinformatics

• Gene Symbol: FOSL1
• Entrez:
  • 8061(Human)
• Uniprot:
  • P15407()