Jumonji/JARID2 Antibody

Western blot shows lysates of IMR-32 human neuroblastoma cell line and Neuro-2A mouse neuroblastoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse Jumonji/JARID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6090) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Jumonji/JARID2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Jumonji/JARID2 Antibody Summary

• Immunogen: E. coli-derived recombinant human Jumonji/JARID2
  Met1-Thr159
  Accession # Q92833
• Specificity: Detects human and mouse Jumonji/JARID2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human JARID2B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: JARID2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jumonji/JARID2 Antibody

• JARID2
• JMJ
• JMJjumonji-like protein
• jumonji (mouse) homolog
• jumonji homolog
• Jumonji
• jumonji, AT rich interactive domain 2
• Jumonji/ARID domain-containing protein 2
• protein Jumonji

Background

Jumonji [Japanese for "cruciform/cross" also JARID2 (Jumonji/ARID domain‑containing protein 2) and JMJ] is a 140‑155 kDa member of the JMJ family of proteins. It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription. Jumonji is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes. Human Jumonji is 1246 amino acids (aa) in length. It contains an NLS (aa 1‑131), a transcriptional repressor domain (aa 132‑222), a DNA binding region (aa 558‑709) and a nonfunctional histone demethylase domain (aa 916‑1042). There are potential splice variants. One shows an alternative start site at Met173, another shows a deletion of aa 304‑1151, and a third contains a 22 aa substitution for aa 1‑60, coupled to a 50 aa substitution for aa 949‑1246. Over aa 1‑159, human Jumonji shares 96% aa identity with mouse Jumonji.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jumonji/JARID2 Antibody (AF6090)(2)

Publications using AF6090

• MK Evans, Y Matsui, B Xu, C Willis, J Loome, L Milburn, Y Fan, V Pagala, JC Peng Ybx1 fine-tunes PRC2 activities to control embryonic brain development Nat Commun, 2020-08-13;11(1):4060. 2020-08-13 [PMID: 32792512] (CyTof, Immunoprecipitation, Mouse)
• Hamza Celik, Won Kyun Koh, Ashley C. Kramer, Elizabeth L. Ostrander, Cates Mallaney, Daniel A.C. Fisher et al. JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells Cancer Cell 11/1/2018 [PMID: 30423295]

Bioinformatics

• Gene Symbol: JARID2
• Uniprot:
  • Q92833(Human)