Jumonji/JARID2 Antibody [Alexa Fluor® 350]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Jumonji/JARID2 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Jumonji/JARID2
  Met1-Thr159
  Accession # Q92833
• Specificity: Detects human and mouse Jumonji/JARID2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human JARID2B is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jumonji/JARID2 Antibody [Alexa Fluor® 350]

• JARID2
• JMJ
• JMJjumonji-like protein
• jumonji (mouse) homolog
• jumonji homolog
• Jumonji
• jumonji, AT rich interactive domain 2
• Jumonji/ARID domain-containing protein 2
• protein Jumonji

Background

Jumonji [Japanese for "cruciform/cross" also JARID2 (Jumonji/ARID domain‑containing protein 2) and JMJ] is a 140‑155 kDa member of the JMJ family of proteins. It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription. Jumonji is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes. Human Jumonji is 1246 amino acids (aa) in length. It contains an NLS (aa 1‑131), a transcriptional repressor domain (aa 132‑222), a DNA binding region (aa 558‑709) and a nonfunctional histone demethylase domain (aa 916‑1042). There are potential splice variants. One shows an alternative start site at Met173, another shows a deletion of aa 304‑1151, and a third contains a 22 aa substitution for aa 1‑60, coupled to a 50 aa substitution for aa 949‑1246. Over aa 1‑159, human Jumonji shares 96% aa identity with mouse Jumonji.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.