JNK1 [p Thr183] Antibody

Images

 
Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), using SAPK/JNK (Phospho-Thr183) Antibody
Immunohistochemistry: JNK1 [p Thr183] Antibody [NB100-82009] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK (phospho-Thr183) antibody.
Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using SAPK/JNK(Phospho-Thr183) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

JNK1 [p Thr183] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human JNK2/3/SAPK around the phosphorylation site of threonine 183 (M-M-TP-P-Y).
Modification
p Thr183
Specificity
SAPK/JNK (specific to Phospho-Thr183) detects endogenous levels of SAPK/JNK only when phosphorylated at threonine 183.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
This antibody is useful for Immunohistochemistry-Paraffin 1:50-1:100. Optimal dilutions should be determined by end user.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-82009.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS [pH 7.4] 150mM NaCl and 50% glycerol (without Mg2+ and Ca2+)
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JNK1 [p Thr183] Antibody

  • c-Jun N-terminal kinase 1
  • EC 2.7.11
  • EC 2.7.11.24
  • JNK1 alpha protein kinase
  • JNK1 beta protein kinase
  • JNK1
  • JNK1JNK1A2
  • JNK-46
  • JUN N-terminal kinase
  • MAP kinase 8
  • MAPK 8
  • MAPK8
  • mitogen-activated protein kinase 8 isoform JNK1 alpha1
  • mitogen-activated protein kinase 8 isoform JNK1 beta2
  • mitogen-activated protein kinase 8
  • PRKM8
  • PRKM8JNK
  • protein kinase JNK1
  • SAPK1
  • SAPK1JNK21B1/2
  • Stress-activated protein kinase 1
  • Stress-activated protein kinase JNK1

Background

JNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JNK1 Antibody (NB100-82009)(2)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAPK8
Entrez
Uniprot