JNK1 [p Thr183] Antibody - BSA Free

Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).

Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), using SAPK/JNK (Phospho-Thr183) Antibody

Immunohistochemistry: JNK1 [p Thr183] Antibody [NB100-82009] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82009

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

JNK1 [p Thr183] Antibody - BSA Free Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human JNK1 around the phosphorylation site of threonine 183 (M-M-TP-P-Y).
• Modification: p Thr183
• Specificity: Detects endogenous levels of JNK1 only when phosphorylated at threonine 183.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Application Notes: This antibody is useful for Immunohistochemistry-Paraffin 1:50-1:100. Optimal dilutions should be determined by end user.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for JNK1 [p Thr183] Antibody - BSA Free

• c-Jun N-terminal kinase 1
• EC 2.7.11
• EC 2.7.11.24
• JNK1 alpha protein kinase
• JNK1 beta protein kinase
• JNK1
• JNK1JNK1A2
• JNK-46
• JUN N-terminal kinase
• MAP kinase 8
• MAPK 8
• MAPK8
• mitogen-activated protein kinase 8 isoform JNK1 alpha1
• mitogen-activated protein kinase 8 isoform JNK1 beta2
• mitogen-activated protein kinase 8
• PRKM8
• PRKM8JNK
• protein kinase JNK1
• SAPK1
• SAPK1JNK21B1/2
• Stress-activated protein kinase 1
• Stress-activated protein kinase JNK1

Background

JNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JNK1 Antibody (NB100-82009)(2)

Publications using NB100-82009

• Wang PX, Ji YX, Zhang XJ et al. Targeting CASP8 and FADD-like apoptosis regulator ameliorates nonalcoholic steatohepatitis in mice and nonhuman primates. Nat. Med. 2017-02-20 [PMID: 28218919]
• Gao L, Wang PX, Zhang Y et al. Tumor necrosis factor receptor-associated factor 5 (Traf5) acts as an essential negative regulator of hepatic steatosis. J Hepatol 2016-07-01 [PMID: 27032381]

Bioinformatics

• Gene Symbol: MAPK8
• Entrez:
  • 5599(Human)
  • 26419(Mouse)
  • 116554(Rat)
• Uniprot:
  • P45984/P53779(Human)