| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB17761-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1776-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71256 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16148-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22209-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82009-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21529-50ul | |||
![Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Western-Blot-NBP1-48284-img0001.jpg "Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] Staining of COS7 cells transiently transfected by pCMV6-ENTRY JNK1.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48284-img0008.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human pancreas tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] - Immunofluorescent staining of HeLa cells using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48284-img0002.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0003.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human Kidney tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human liver tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0005.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human Ovary tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0006.jpg)
![Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.](http://images.novusbio.com/fullsize/JNK1-Antibody-Western-Blot-NB100-1925-img0004.jpg "Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.")
![Western Blot: JNK1/2/3 Antibody [NBP3-16148] - Western blot analysis of extracts of various cell lines, using JNK1/2/3 antibody (NBP3-16148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/JNK1-2-3-Antibody-Western-Blot-NBP3-16148-img0002.jpg "Western Blot: JNK1/2/3 Antibody [NBP3-16148] - Western blot analysis of extracts of various cell lines, using JNK1/2/3 antibody (NBP3-16148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunoprecipitation: JNK1/2/3 Antibody [NBP3-16148] - Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug JNK1/2/3 antibody (NBP3-16148). Western blot was performed from the immunoprecipitate using JNK1/2/3 antibody (NBP3-16148) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/JNK1-2-3-Antibody-Immunoprecipitation-NBP3-16148-img0003.jpg)
![Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded mouse spleen using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-5220251255553.jpg)
![Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded human colon using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-522025125443.jpg)
![Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded mouse brain using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-52202511392252.jpg)
![Immunocytochemistry/ Immunofluorescence: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunofluorescence analysis of U2OS cells using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-52202511361224.jpg)
![Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody (M11), clone 2F11. Lane 1: MAPK8 transfected lysatE (48.3 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Western-Blot-H00005599-M11-img0010.jpg "Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody (M11), clone 2F11. Lane 1: MAPK8 transfected lysatE (48.3 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of monoclonal antibody to MAPK8 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Immunocytochemistry-Immunofluorescence-H00005599-M11-img0005.jpg)
![Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 over-expressed 293 cell line, cotransfected with MAPK8 Validated Chimera RNAi or non-transfected control. Blot probed with H00005599-M11. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Western-Blot-H00005599-M11-img0002.jpg)
![Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of protein-protein interactions between CRK and MAPK8. HeLa cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Proximity-Ligation-Assay-H00005599-M11-img0006.jpg)
![Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of protein-protein interactions between PXN and MAPK8. Huh7 cells were stained with anti-PXN rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Proximity-Ligation-Assay-H00005599-M11-img0007.jpg)
![Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of protein-protein interactions between BAD and MAPK8. Mahlavu cells were stained with anti-BAD rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Proximity-Ligation-Assay-H00005599-M11-img0008.jpg)
![Sandwich ELISA: JNK1 Antibody (2F11) [H00005599-M11] - Detection limit for recombinant GST tagged MAPK8 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Sandwich-ELISA-H00005599-M11-img0009.jpg)
![Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using JNK1 mouse mAb against A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate.](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-Western-Blot-NBP2-22209-img0004.jpg "Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using JNK1 mouse mAb against A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate.")
![Flow Cytometry: JNK1 Antibody (1E5) [NBP2-22209] - Flow cytometric analysis of HeLa cells using JNK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-Flow-Cytometry-NBP2-22209-img0001.jpg)
![Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using MAPK8 mAb against human JNK1 (AA: 227-380) recombinant protein. (Expected MW is 43.4 kDa)](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-Western-Blot-NBP2-22209-img0003.jpg)
![ELISA: JNK1 Antibody (1E5) [NBP2-22209] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-ELISA-NBP2-22209-img0002.jpg)
![Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Western-Blot-NBP3-16536-img0005.jpg "Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of U-2 OS cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunohistochemistry of paraffin-embedded human colon using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunohistochemistry-Paraffin-NBP3-16536-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of C6 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of NIH-3T3 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0003.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/JNK1-p-Thr183-p-Tyr185-Antibody-HL1008-Western-Blot-NBP3-13678-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - JNK1 (phospho Thr183/Tyr185) antibody [HL1008] detects JNK1 (phospho Thr183/Tyr185) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. JNK1 (phospho Thr183/Tyr185) stained by JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/JNK1-p-Thr183-p-Tyr185-Antibody-HL1008-Immunohistochemistry-Paraffin-NBP3-13678-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/JNK1-p-Thr183-p-Tyr185-Antibody-HL1008-Western-Blot-NBP3-13678-img0001.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-9102024204127.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) Raw264.7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-91020242013406.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) PC-12 whole cell extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-91020241959351.jpg)
![Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Western-Blot-NB100-82009-img0001.jpg "Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).")
![<b>Biological Strategies Validation. </b>Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), using SAPK/JNK (Phospho-Thr183) Antibody](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Western-Blot-NB100-82009-img0003.jpg)
![Immunohistochemistry: JNK1 [p Thr183] Antibody [NB100-82009] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82009](http://images.novusbio.com/fullsize/nb100-82009_rabbit-polyclonal-jnk1-p-thr183-antibody-103202523464547.jpg)
![Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-572023156479.jpg "Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.")
![Immunohistochemistry: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunohistochemical analysis of paraffin-embedded human breast, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-5720231558484.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-6720231051264.jpg)
![Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-27820241631699.jpg "Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32487) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-29820241435284.jpg)
![Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunocytochemistry analysis of HEK-293 cells labeling JNK1 with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-2920241192320.jpg)