JMJD2D Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: JMJD2D Antibody [NBP2-47413] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: JMJD2D Antibody [NBP2-47413] - Staining of human testis shows moderate nuclear positivity in subset of cells in seminiferus ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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Catalog# & Formulation Size Price

JMJD2D Antibody - BSA Free Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LRQLPSHWARHSPWPMAARSGTRCHTLVCSSLPRRSAVSGTATQPRAAAVHSSKKPSSTPSSTPGPSAQIIHPSN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM4D
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
JMJD2D Protein (NBP2-47413PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for JMJD2D Antibody - BSA Free

  • EC 1.14.11
  • EC 1.14.11.-
  • FLJ10251
  • JHDM3D
  • JmjC domain-containing histone demethylation protein 3D
  • JMJD2D
  • JMJD2Dlysine-specific demethylase 4D
  • jumonji domain containing 2D
  • Jumonji domain-containing protein 2D
  • KDM4D
  • lysine (K)-specific demethylase 4D
  • MGC141909

Background

JMJD2 is a member of the family of novel JmjC domain-containing histone demethylation (JHDM) enzymes, which has been shown to perform the enzymatic removal of methyl groups from histones. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Histone demethylase activity has been linked to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KDM4D