JMJD1C Antibody - Azide and BSA Free

Western Blot: JMJD1C Antibody [NBP3-15959] - Western blot analysis of extracts of various cell lines, using JMJD1C antibody (NBP3-15959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: JMJD1C Antibody [NBP3-15959] - Immunohistochemistry of paraffin-embedded mouse spinal cord using JMJD1C Rabbit pAb (NBP3-15959) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: JMJD1C Antibody [NBP3-15959] - Immunohistochemistry of paraffin-embedded rat brain using JMJD1C Rabbit pAb (NBP3-15959) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: JMJD1C Antibody [NBP3-15959] - Immunohistochemistry of paraffin-embedded human esophageal using JMJD1C Rabbit pAb (NBP3-15959) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

JMJD1C Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 354-448 of human JMJD1C (NP_116165.1). KPEEDEKKLNMKRLRTDNVSDFSESSDSENSNKRIIDNSSEQKPENELKNKNTSKINGEEGKPHNNEKAGEETLKNSQPPWDQIQEDKKHEEAEK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: JMJD1C
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for JMJD1C Antibody - Azide and BSA Free

• DKFZp761F0118
• EC 1.14.11
• EC 1.14.11.-
• FLJ14374
• JHDM2C
• jumonji domain containing 1C
• Jumonji domain-containing protein 1C
• KIAA1380TR-interacting protein 8
• probable JmjC domain-containing histone demethylation protein 2C
• RP11-10C13.2
• thyroid hormone receptor interactor 8
• thyroid receptor interacting protein 8
• Thyroid receptor-interacting protein 8
• TRIP8
• TRIP-8

Background

JMJD1C is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. Some recently characterized members of the jmj family include JARID1A/RBP2, JARID1C, JMJD1A, JMJD1B, JMJD1C, JMJD2A, JMJD2C and JMJD2D. JMJD1C has been identified as a nuclear-receptor coactivator and is a candidate gene for autism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: JMJD1C