JMJD1B Antibody

Images

 
Western Blot: JMJD1B Antibody [NBP1-77071] - Rat liver tissue lysate with JMJD1B antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: JMJD1B Antibody [NBP1-77071] - Immunofluorescence of JMJD1B in rat liver tissue with JMJD1B antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: JMJD1B Antibody [NBP1-77071] - Rat liver tissue with JMJD1B antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

JMJD1B Antibody Summary

Immunogen
Antibody was raised against a 20 amino acid synthetic peptide from near the center of human JMJD1B. The immunogen is located within amino acids 1050 - 1100 of JMJD1B.
Specificity
This antibody will not cross-react with JMJD1A or JMJD1C.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
JMJD1B Peptide (NBP1-77071PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77071 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JMJD1B Antibody

  • C5orf7
  • EC 1.14.11
  • EC 1.14.11.-
  • JHDM2B
  • JmjC domain-containing histone demethylation protein 2B
  • JMJD1B
  • jumonji domain containing 1B
  • Jumonji domain-containing protein 1B
  • KIAA1082chromosome 5 open reading frame 7
  • lysine (K)-specific demethylase 3B
  • lysine-specific demethylase 3B
  • NET22,5qNCA
  • Nuclear protein 5qNCA

Background

The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist. This antibody will not cross-react with JMJD1A or JMJD1C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for JMJD1B Antibody (NBP1-77071) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-77071:
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 09/07/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for JMJD1B Antibody (NBP1-77071) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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2
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1
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Anonymous
09/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol KDM3B
Entrez
Uniprot