JMJD1B Antibody - BSA Free

Images

 
Western Blot: JMJD1B Antibody [NBP1-77071] - Rat liver tissue lysate with JMJD1B antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: JMJD1B Antibody - BSA Free [NBP1-77071] - Immunofluorescence of JMJD1B in rat liver tissue with JMJD1B antibody at 20 u/mL.
Immunohistochemistry: JMJD1B Antibody - BSA Free [NBP1-77071] - Immunohistochemistry of JMJD1B in rat liver tissue with JMJD1B antibody at 2.5 u/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

JMJD1B Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 20 amino acid synthetic peptide from near the center of human JMJD1B. The immunogen is located within amino acids 1050 - 1100 of JMJD1B. Amino Acid Squence: YRLRKSRPRSETEEMGDEE
Specificity
This antibody will not cross-react with JMJD1A or JMJD1C.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM3B
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
Control Peptide
JMJD1B Peptide (NBP1-77071PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77071 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for JMJD1B Antibody - BSA Free

  • C5orf7
  • EC 1.14.11
  • EC 1.14.11.-
  • EC 1.14.11.65
  • JHDM2B
  • JmjC domain-containing histone demethylation protein 2B
  • JMJD1B
  • jumonji domain containing 1B
  • Jumonji domain-containing protein 1B
  • KDM3B
  • KIAA1082
  • KIAA1082chromosome 5 open reading frame 7
  • lysine (K)-specific demethylase 3B
  • lysine-specific demethylase 3B
  • NET22,5qNCA
  • Nuclear protein 5qNCA

Background

The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist. This antibody will not cross-react with JMJD1A or JMJD1C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JMJD1B Antibody (NBP1-77071) (0)

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Review for JMJD1B Antibody (NBP1-77071) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-77071:
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WB
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Human 09/07/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Verified Customer
09/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol KDM3B
Entrez
Uniprot