JAM-A Antibody (H202-106) - BSA Free

Immunohistochemistry: JAM-A Antibody (H202-106) [NB100-65340] - Mouse proximal colon tissue section. Antibody at 1:200 at 4C overnight. 40X magnification. IHC-Fr image submitted by a verified customer review. Image using the Alexa Fluor 488 format of this antibody.

Flow Cytometry: JAM-A Antibody (H202-106) [NB100-65340] - Analysis using the Biotin conjugate of NB100-65340. Staining of mouse peripheral blood platelets.

Flow Cytometry: JAM-A Antibody (H202-106) [NB100-65340] - Staining of mouse peripheral blood platelets with RAT ANTI MOUSE CD321.

• Reactivity: Mu
• Applications: Flow, IHC, IP
• Clone: H202-106
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

JAM-A Antibody (H202-106) - BSA Free Summary

• Description: Novus Biologicals Rat JAM-A Antibody (H202-106) - BSA Free (NB100-65340) is a monoclonal antibody validated for use in IHC, Flow and IP. Anti-JAM-A Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: MTE1/MTE2 stromal cell lines
• Localization: Type I membrane protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: F11R
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for JAM-A Antibody (H202-106) - BSA Free

• CD321 antigen
• CD321
• F11 receptor
• F11R
• JAM-1
• JAM1CD321
• JAMA
• JAM-A
• JCAMJAM
• Junctional adhesion molecule 1Platelet F11 receptor
• junctional adhesion molecule A
• PAM-1
• PAM-1KAT
• Platelet adhesion molecule 1

Background

Junctional Adhesion Molecule 1 (JAM1) seems to plays a role in epithelial tight junction formation. It appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. JAM1 plays a role in regulating monocyte transmigration involved in integrity of the epithelial barrier. JAM1 is also involved in platelet activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JAM-A Antibody (NB100-65340)(4)

Publications using NB100-65340

• Prestwich, RJ et al. Immune-mediated antitumor activity of reovirus is required for therapy and is independent of direct viral oncolysis and replication Clin Cancer Res 2009-01-01 [PMID: 19509134]
  
  Details:
  This citation used the Biotin version of this antibody.
• Aurrand-Lions, M et al. Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members Blood 2001-01-01 [PMID: 11739175]
  
  Details:
  This citation used the Biotin version of this antibody.
• Ebnett, Ket al. Junctional Adhesion Molecule Interacts with the PDZ Domain-containing Proteins AF-6 and ZO-1 J Biol Chem 2000-01-01 [PMID: 10856295]
  
  Details:
  This citation used the Biotin version of this antibody.
• Malergue, Fet al. A novel immunoglobulin superfamily junctional molecule expressed by antigen presenting cells, endothelial cells and platelets Mol Immunol 1998-01-01 [PMID: 10395200]
  
  Details:
  This citation used the Biotin version of this antibody.

Bioinformatics

• Gene Symbol: F11R
• Entrez:
  • 16456(Mouse)
• Uniprot:
  • O88792()
  • O88792()