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1077-JM) was serially diluted and captured by Goat Anti-Mouse JAM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1077) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse JAM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1077) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
1077-JM) was serially diluted and captured by Goat Anti-Mouse JAM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1077) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse JAM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1077) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
NL003) and counterstained with DAPI (blue). Specific staining was localized to intercellular junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.