JAM-A Antibody (H202-106) [Alexa Fluor® 488]

Immunohistochemistry-Frozen: JAM-A Antibody (H202-106) [Alexa Fluor® 488] [NB100-65340AF488] - Mouse proximal colon tissue section. Antibody at 1:200 at 4C overnight. 40X magnification. IHC-Fr image submitted by a verified customer review.

• Reactivity: Mu
• Applications: Flow, IHC, IP
• Clone: H202-106
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

JAM-A Antibody (H202-106) [Alexa Fluor® 488] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: MTE1/MTE2 stromal cell lines
• Localization: Type I membrane protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: F11R
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB100-65340. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50 mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for JAM-A Antibody (H202-106) [Alexa Fluor® 488]

• CD321 antigen
• CD321
• F11 receptor
• F11R
• JAM-1
• JAM1CD321
• JAMA
• JAM-A
• JCAMJAM
• Junctional adhesion molecule 1Platelet F11 receptor
• junctional adhesion molecule A
• PAM-1
• PAM-1KAT
• Platelet adhesion molecule 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for JAM-A Antibody (NB100-65340AF488) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 12/19/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: proximal colon PFA 4% fixation,proximal colon
• Species: Mouse
• Lot: 148182-110619AF488

Comments

• Comments: staining incubation over night +4, dilution 1/200

Bioinformatics

• Gene Symbol: F11R