Jak2 Antibody

Western blot shows lysates of DA3 mouse myeloma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell lines. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Jak2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2988) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jak2 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Jak2 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse Jak2
  Gly2-Val63
  Accession # Q62120
• Specificity: Detects endogenous mouse and rat Jak2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: JAK2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is sold under license from Millipore Corporation under the following US or foreign patents: 5,821,069; 5,658,791; EP0560890. This product shall not be used to commercially screen drug molecules being developed as JAK1 or JAK2 inhibitors. Any such activity will be outside the scope of the research use only label license.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jak2 Antibody

• EC 2.7.10
• EC 2.7.10.2
• Jak2
• JAK-2
• Janus kinase 2Janus kinase 2 (a protein tyrosine kinase)
• JTK10
• tyrosine-protein kinase JAK2

Background

Janus Kinase 2 (Jak2) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak2 and other family members Jak1, Jak3, and Tyk2 occurs via phosphorylation at two adjacent tyrosine residues as well as potentially one or more serine residues. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak2 plays an essential role in interferon gamma signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jak2 Antibody (AF2988)(2)

Publications using AF2988

• YQ Zheng, YR Cui, S Yang, YP Wang, YJ Qiu, WL Hu Opa interacting protein 5 promotes metastasis of nasopharyngeal carcinoma cells by promoting EMT via modulation of JAK2/STAT3 signal Eur Rev Med Pharmacol Sci, 2019-01-01;23(2):613-621. 2019-01-01 [PMID: 30720169] (Western Blot, Human)
• Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt. Apoptosis, 2010-04-01;15(4):463-73. 2010-04-01 [PMID: 19997978] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: JAK2
• Uniprot:
  • Q62120()