Jak1 [p Tyr1022] Antibody

Western Blot: Jak1 [p Tyr1022] Antibody [NB100-82005] - Analysis of extracts from HT29 cells untreated or treated with UV using JAK1 [p Tyr1022] antibody.

Immunohistochemistry-Paraffin: Jak1 [p Tyr1022] Antibody [NB100-82005] - Analysis of paraffin-embedded human breast carcinoma tissue, using JAK1 (phospho-Tyr1022) antibody

Western Blot: Jak1 [p Tyr1022] Antibody [NB100-82005] - Analysis of extract from thyroid cancer cell line BHP cells (BHP 5, 14, 15 and 17), using JAK1 (phospho-Tyr1022) antibody. Only BHP 5, 15 and 17 can detect the phosphorylated JAK1 protein at the molecular weight of 130kD.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Jak1 [p Tyr1022] Antibody Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human JAK1 around the phosphorylation site of tyrosine 1022 (K-E-YP-Y-T).
• Modification: p Tyr1022
• Specificity: Detects endogenous levels of Jak1 only when phosphorylated at tyrosine 1022.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: JAK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Jak1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Jak1 [p Tyr1022] Antibody

• EC 2.7.10
• Jak1
• JAK-1
• JAK1Atyrosine-protein kinase JAK1
• JAK1B
• Janus kinase 1EC 2.7.10.2
• JTK3

Background

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterised by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signalling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jak1 Antibody (NB100-82005)(1)

Publication using NB100-82005

• Al-Ani B, ShamsEldeen AM, Kamar SS et al. Lipopolysaccharide induces acute lung injury and alveolar hemorrhage in association with the cytokine storm, coagulopathy and AT1R/JAK/STAT augmentation in a rat model that mimics moderate and severe Covid-19 pathology Clinical and experimental pharmacology & physiology 2022-01-23 [PMID: 35066912] (WB, Rat)

Bioinformatics

• Gene Symbol: JAK1
• Entrez:
  • 3716(Human)
  • 16451(Mouse)
  • 84598(Rat)
• Uniprot:
  • P23458()