Jak1 [p Tyr1022] Antibody

Images

 
Western Blot: JAK1 [p Tyr1022] Antibody [NB100-82005] - Analysis of extracts from HT29 cells untreated or treated with UV using JAK1 [p Tyr1022] antibody.
Immunohistochemistry-Paraffin: Jak1 [p Tyr1022] Antibody [NB100-82005] - Analysis of paraffin-embedded human breast carcinoma tissue, using JAK1 (phospho-Tyr1022) antibody
Western Blot: JAK1 [p Tyr1022] Antibody [NB100-82005] - Analysis of extract from thyroid cancer cell line BHP cells (BHP 5, 14, 15 and 17), using JAK1 (phospho-Tyr1022) antibody. Only BHP 5, 15 and 17 can detect the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Jak1 [p Tyr1022] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human JAK1 around the phosphorylation site of tyrosine 1022 (K-E-YP-Y-T).
Modification
p Tyr1022
Specificity
JAK1 (specific to Phospho-Tyr1022) detects endogenous levels of JAK1 only when phosphorylated at tyrosine 1022.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
JAK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jak1 Lysate (NBL1-12093)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Jak1 [p Tyr1022] Antibody

  • EC 2.7.10
  • Jak1
  • JAK-1
  • JAK1Atyrosine-protein kinase JAK1
  • JAK1B
  • Janus kinase 1EC 2.7.10.2
  • JTK3

Background

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterised by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signalling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Jak1.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

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Bioinformatics

Gene Symbol JAK1
Entrez
Uniprot