ITPKA Antibody

Images

 
Western Blot: ITPKA Antibody [NBP1-81590] - Analysis in human cerebral cortex tissue.
Immunocytochemistry/ Immunofluorescence: ITPKA Antibody [NBP1-81590] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ITPKA Antibody [NBP1-81590] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry: ITPKA Antibody [NBP1-81590] - Staining of mouse hippocampus shows positivity in astrocytes.
Immunohistochemistry: ITPKA Antibody [NBP1-81590] - Staining of mouse hypothalamus shows positivity in astrocyte end feet accentuated around blood vessels.
Immunohistochemistry: ITPKA Antibody [NBP1-81590] - Staining of mouse subiculum shows immunoreactivity in astrocytes.
Immunohistochemistry-Paraffin: ITPKA Antibody [NBP1-81590] - Staining of human caudate shows strong positivity in astrocytes.
Immunohistochemistry-Paraffin: ITPKA Antibody [NBP1-81590] - Staining of human cerebral cortex shows moderate positivity in astrocytes.
Immunohistochemistry-Paraffin: ITPKA Antibody [NBP1-81590] - Staining of human glioma shows strong cytoplasmic positivity in tumor cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ITPKA Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DGSCSTDFKTTRSREQVLRVFEEFVQGDEEVLRRYLNRLQQIRDTLEVSEFFRRHEV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ITPKA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ITPKA Protein (NBP1-81590PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ITPKA Antibody

  • EC 2.7.1.127
  • inositol 14,5-trisphosphate 3-kinase AIP3 3-kinase A
  • inositol-trisphosphate 3-kinase A
  • InsP 3-kinase A
  • IP3-3KA
  • IP3K A
  • IP3KA

Background

Regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro. ITPKA and ITPKB are 68% identical in the C-terminus region. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ITPKA