ITM2C Overexpression Lysate

Images

 
Western Blot: ITM2C Overexpression Lysate (Adult Normal) [NBL1-12085] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ITM2C.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ITM2C Overexpression Lysate Summary

Description

ITM2C Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC206403

Accession#: NM_001012516

Protein Tag: C-MYC/DDK
Gene
ITM2C

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ITM2C Overexpression Lysate

  • BRI3
  • BRI3E25C
  • BRICD2C
  • BRICHOS domain containing 2C
  • Cerebral protein 14
  • E25
  • hRPC.1050_D_4
  • integral membrane protein 2C
  • integral membrane protein 3
  • ITM2C
  • ITM3
  • Transmembrane protein BRI3

Background

ITM2C also known as Integral membrane protein 2C, has 3 isoforms, an isoform 1that is 267 amino acid and 30 kDa, an isoform 2 that is 220 amino acid and 25 kDa, and an isoform 3 that is 230 amino acid and 26 kDa; most commonly found in the brain tissue; acts as negative regulator of beta amyloid peptide production, may inhibit the processing of APP by blocking its access to alpha- and beta-secretase, appears to be a poor gamma-secretase cleavage inhibitor, and may play a role in TNF-induced cell death and neuronal differentiation. Studies are being performed on the relationship of this protein to anemia of prematurity, cerebritis, anemia, dementia, influenza, gastric cancer, and neuronitis. The protein has been shown to interact with BACE1, APP, STMN2, STT3A, RNF7, and FURIN proteins in negative regulation of neuron projection development, induction of programmed cell death, and neuron differentiation pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-36963
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
AF4876
Species: Hu
Applications: WB
DY413
Species: Mu
Applications: ELISA
AF2377
Species: Mu
Applications: IHC, Simple Western, WB
NBP2-13075
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-87201
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-22261
Species: Ch, Hu, Mu
Applications: IP, WB
NB500-106
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
NBP2-16686
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-49118
Species: Hu
Applications: IHC, IHC-P
NBP2-31688
Species: Hu
Applications: IHC, IHC-P
NB120-11427
Species: Fe, Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
NBP3-16473
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NBP2-50465
Species: Hu
Applications: CyTOF-ready, Flow-CS, Flow, IHC, IHC-P
NBP2-42864
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-59510
Species: Hu
Applications: IHC, IHC-P, WB
MAB1419
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
210-TA
Species: Hu
Applications: BA
936-AD
Species: Hu
Applications: EnzAct
NBL1-12085
Species: Hu
Applications: WB

Publications for ITM2C Lysate (NBL1-12085) (0)

There are no publications for ITM2C Lysate (NBL1-12085).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ITM2C Lysate (NBL1-12085) (0)

There are no reviews for ITM2C Lysate (NBL1-12085). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ITM2C Lysate (NBL1-12085) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ITM2C Products

Array NBL1-12085

Bioinformatics Tool for ITM2C Lysate (NBL1-12085)

Discover related pathways, diseases and genes to ITM2C Lysate (NBL1-12085). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ITM2C Lysate (NBL1-12085)

Discover more about diseases related to ITM2C Lysate (NBL1-12085).
 

Pathways for ITM2C Lysate (NBL1-12085)

View related products by pathway.

Research Areas for ITM2C Lysate (NBL1-12085)

Find related products by research area.

Blogs on ITM2C

There are no specific blogs for ITM2C, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ITM2C Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol ITM2C