Isopeptidase T/USP5 Antibody

Images

 
Western Blot: Isopeptidase T/USP5 Antibody [NBP1-32590] - Sample(30 ug whole cell lysate)A:MOLT4 B:Raji 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NBP1-32590] - Paraffin-embedded TOV-112D xenograft, using antibody at 1:500 dilution.
Western Blot: Isopeptidase T/USP5 Antibody [NBP1-32590] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.81 mg/ml

Order Details

Isopeptidase T/USP5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human USP5. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.81 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Isopeptidase T/USP5 Antibody

  • Deubiquitinating enzyme 5
  • EC 3.1.2.15
  • EC 3.4.19.12
  • Isopeptidase T
  • IsoT
  • ISOTubiquitin carboxyl-terminal hydrolase 5
  • ubiquitin isopeptidase T
  • ubiquitin specific peptidase 5 (isopeptidase T)
  • ubiquitin specific protease 5 (isopeptidase T)
  • ubiquitin thioesterase 5
  • Ubiquitin thiolesterase 5
  • ubiquitin-specific protease-5 (ubiquitin isopeptidase T)
  • Ubiquitin-specific-processing protease 5
  • USP5

Background

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol USP5
Entrez