Isopeptidase T/USP5 Antibody - BSA Free

Western Blot: Isopeptidase T/USP5 Antibody [NBP1-32590] - Sample(30 ug whole cell lysate)A:MOLT4 B:Raji 7. 5% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NBP1-32590] - Mouse kidney. USP5 antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Isopeptidase T/USP5 Antibody [NBP1-32590] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NBP1-32590] - TOV-112D xenograft, using USP5 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Isopeptidase T/USP5 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Isopeptidase T/USP5. The exact sequence is proprietary.
• Predicted Species: Rat (99%), Rhesus Macaque (92%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Isopeptidase T/USP5 Antibody - BSA Free

• Deubiquitinating enzyme 5
• EC 3.1.2.15
• EC 3.4.19.12
• Isopeptidase T
• IsoT
• ISOTubiquitin carboxyl-terminal hydrolase 5
• ubiquitin isopeptidase T
• ubiquitin specific peptidase 5 (isopeptidase T)
• ubiquitin specific protease 5 (isopeptidase T)
• ubiquitin thioesterase 5
• Ubiquitin thiolesterase 5
• ubiquitin-specific protease-5 (ubiquitin isopeptidase T)
• Ubiquitin-specific-processing protease 5
• USP5

Background

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: USP5