USP13 Antibody (OTI4G11)

Western Blot: USP13 Antibody (OTI4G11) [NBP1-48047] - Analysis of extracts (35ug) from 9 different cell lines by using anti-USP13 monoclonal antibody.

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded prostate tissue using anti-USP13 mouse monoclonal antibody.

Western Blot: USP13 Antibody (OTI4G11) [NBP1-48047] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP13 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP13.

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded breast tissue using anti-USP13 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-USP13 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded colon tissue using anti-USP13 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded Kidney tissue using anti-USP13mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded liver tissue using anti-USP13 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded lung tissue using anti-USP13 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded Ovary tissue using anti-USP13 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded pancreas tissue using anti-USP13 mouse monoclonal antibody.

Immunoprecipitation: USP13 Antibody (OTI4G11) [NBP1-48047] - (Negative control: IP without adding anti-USP13 antibody.). For each experiment, 500ul of DDK tagged USP13 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-USP13 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, IHC, IP
• Clone: OTI4G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.41 mg/ml

USP13 Antibody (OTI4G11) Summary

• Description: Novus Biologicals Mouse USP13 Antibody (OTI4G11) (NBP1-48047) is a monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human USP13 expression vector
• Specificity: This antibody is specific for Homo sapiens ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 4ug/ml
  • Western Blot 1:1000
• Theoretical MW: 97.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.41 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP13 Antibody (OTI4G11)

• Deubiquitinating enzyme 13
• EC 3.1.2.15
• EC 3.4.19.12
• Isopeptidase T-3
• IsoT-3
• ISOT3IsoT-3
• ubiquitin carboxyl-terminal hydrolase 13
• ubiquitin specific peptidase 13 (isopeptidase T-3)
• ubiquitin specific protease 13 (isopeptidase T-3)
• Ubiquitin Thioesterase 13
• Ubiquitin thiolesterase 13
• Ubiquitin-specific-processing protease 13
• USP13

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP13
• Entrez:
  • 8975(Human)