Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate from HeLa, (5, 15 and 50 ug), and 293T (15ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody used for WB ...read more
Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit antiLasu1/Ureb1used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP) from 293T cells. Ureb1/Lasu1 was less efficiently immunoprecipitated by another Ureb1/Lasu1 antibody.
Proximity Ligation Assay: HUWE1 Antibody [NB100-652] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human Lasu1 (NB100-652). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: HUWE1 Antibody [NB100-652] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human USP7 (NBP2-22295) and rabbit anti-human Lasu1 (NB100-652). Image merged from DAPI (2ms), ...read more
The immunogen this antibody was made to, maps to a region between residues 2250 and 2300 of human Upstream Regulatory Element Binding Protein 1 using the numbering given in entry NP_113584.3 (GeneID 10075).
Immunogen affinity purified
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481 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
FAQs for HUWE1 Antibody (NB100-652). (Showing 1 - 1 of 1 FAQs).
Can you tell me what percentage of gel was used for the Western Blot and what the transfer time and conditions were for product NB100-652.
This product is tested by a collaborator so I do not have the specifics for this antibody in particular at the moment. I have consulted our lab and for a large protein like this (around 480kDa) we recommend either a 5% or 3-8% gradient polyacrylamide gel. Additionally, we recommend that you boil your samples for 10 minutes at 68 degrees and use your standard transfer conditions. If you need a ladder for that high of a molecular weight, we use HiMark from Invitrogen.
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