HUWE1 Antibody


Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP) from 293T cells. Ureb1/Lasu1 was less efficiently immunoprecipitated by another Ureb1/Lasu1 antibody.
Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiLasu1/Ureb1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB more
Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - Human ovarian carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Proximity Ligation Assay: HUWE1 Antibody [NB100-652] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human Lasu1 (NB100-652). Image merged from DAPI (2ms) and Texas more
Proximity Ligation Assay: HUWE1 Antibody [NB100-652] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human USP7 (NBP2-22295) and rabbit anti-human Lasu1 (NB100-652). Image merged from DAPI (2ms), more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, PLA
1.0 mg/ml

Order Details

HUWE1 Antibody Summary

The immunogen this antibody was made to, maps to a region between residues 2250 and 2300 of human Upstream Regulatory Element Binding Protein 1 using the numbering given in entry NP_113584.3 (GeneID 10075).
Predicted Species
Primate (100%), Monkey (100%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate buffer, pH 7 to 8
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


  • Western Blot 1:2000 - 1:10000
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:1000
  • Immunohistochemistry 1:500 to 1:2000
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Immunoprecipitation 2 - 10ug/mg lysate
  • Proximity Ligation Assay 1:10000 - 1:20000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
481 kDa
Read Publications using
NB100-652 in the following applications:

  • 1 publication
  • IP
    2 publications
  • WB
    2 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee and Northern white-cheeked gibbon.

Alternate Names for HUWE1 Antibody

  • EC 6.3.2
  • EC
  • HECT, UBA and WWE domain containing 1
  • HECTH9
  • HSPC272
  • KIAA0312MULE
  • Mcl-1 ubiquitin ligase E3
  • Mule
  • UREB1UBA and WWE domain-containing protein 1
  • URE-binding protein 1


ARFBP1 is a novel E3 ubiquitin ligase. It interacts with p19ARF and its ligase activity is inhibited by this interaction.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HUWE1 Antibody (NB100-652)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-652 Applications Species
Choe KN, Nicolae CM, Constantin D et al. HUWE1 interacts with PCNA to alleviate replication stress. EMBO Rep. May 4 2016 12:00AM [PMID:27146073] (WB, ICC/IF, IP, Human) WB, ICC/IF, IP Human
Rolfo A, Garcia J, Todros T et al. The double life of MULE in preeclamptic and IUGR placentae. Cell Death Dis 2012 May [PMID:22552282]
Noy T, Suad O, Taglicht D, Ciechanover A. HUWE1 ubiquitinates MyoD and targets it for proteasomal degradation. Biochem Biophys Res Commun;418(2):408-13. 2012 Feb 10. [PMID:22277673]
Mandel I, Paperna T, Miller A et al. Aberrant expression of the apoptosis-related proteins BAK and MCL1 in T cells in multiple sclerosis. J Neuroimmunol 2012 Mar [PMID:22257632]
Chanthery YH, Gustafson WC, Itsara M et al. Paracrine signaling through MYCN enhances tumor-vascular interactions in neuroblastoma. Sci Transl Med 2012 Jan [PMID:22218692]
Markkanen E, van Loon B, Ferrari E et al. Regulation of oxidative DNA damage repair by DNA polymerase ? and MutYH by cross-talk of phosphorylation and ubiquitination. Proc Natl Acad Sci U S A 2012 Jan [PMID:22203964]
Kon N, Zhong J, Qiang L et al. Inactivation of arf-bp1 induces p53 activation and diabetic phenotypes in mice. J Biol Chem 2012 Feb [PMID:22187431]
Yamamoto SP, Okawa K, Nakano T et al. Huwe1, a novel cellular interactor of Gag-Pol through integrase binding, negatively influences HIV-1 infectivity Microbes Infect 2011 Apr [PMID:21167302] (IP, WB, Human) IP, WB Human
Schwickart M, Huang X, Lill JR et al. Deubiquitinase USP9X stabilizes MCL1 and promotes tumour cell survival. Nature 2010 Jan [PMID:20023629]
Stankiewicz AR, Livingstone AM, Mohseni N et al. Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70. Cell Death Differ 2009 Apr [PMID:19148187]
Show All 12 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HUWE1 Antibody (NB100-652). (Showing 1 - 1 of 1 FAQs).

  1. Can you tell me what percentage of gel was used for the Western Blot and what the transfer time and conditions were for product NB100-652.
    • This product is tested by a collaborator so I do not have the specifics for this antibody in particular at the moment. I have consulted our lab and for a large protein like this (around 480kDa) we recommend either a 5% or 3-8% gradient polyacrylamide gel. Additionally, we recommend that you boil your samples for 10 minutes at 68 degrees and use your standard transfer conditions. If you need a ladder for that high of a molecular weight, we use HiMark from Invitrogen.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol HUWE1

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