HUWE1 Antibody

Images

 
Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate from HeLa, (5, 15 and 50 ug), and 293T (15ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody used for WB ...read more
Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit antiLasu1/Ureb1used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP) from 293T cells. Ureb1/Lasu1 was less efficiently immunoprecipitated by another Ureb1/Lasu1 antibody.
Proximity Ligation Assay: HUWE1 Antibody [NB100-652] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human Lasu1 (NB100-652). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: HUWE1 Antibody [NB100-652] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human USP7 (NBP2-22295) and rabbit anti-human Lasu1 (NB100-652). Image merged from DAPI (2ms), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

HUWE1 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residues 2250 and 2300 of human Upstream Regulatory Element Binding Protein 1 using the numbering given in entry NP_113584.3 (GeneID 10075).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HUWE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:1000
  • Immunohistochemistry 1:500 to 1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:10000 - 1:20000
Theoretical MW
481 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB100-652 in the following applications:

  • 1 publication
  • IP
    3 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HUWE1 Antibody

  • EC 6.3.2
  • EC 6.3.2.6
  • HECT, UBA and WWE domain containing 1
  • HECTH9
  • HSPC272
  • KIAA0312MULE
  • Mcl-1 ubiquitin ligase E3
  • Mule
  • UREB1UBA and WWE domain-containing protein 1
  • URE-binding protein 1

Background

ARFBP1 is a novel E3 ubiquitin ligase. It interacts with p19ARF and its ligase activity is inhibited by this interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, IP, PLA

Publications for HUWE1 Antibody (NB100-652)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(3)
WB
(2)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-652 Applications Species
Crow MS, Cristea IM. Human antiviral protein IFIX suppresses viral gene expression during HSV-1 infection and is counteracted by virus-induced proteasomal degradation Mol. Cell Proteomics Jan 11 2017 [PMID: 28077445]
Cheng YF, Tong M, Edge AS. Destabilization of Atoh1 by E3 Ubiquitin Ligase Huwe1 and Casein Kinase 1 Is Essential for Normal Sensory Hair Cell Development. J. Biol. Chem. Aug 19 2016 [PMID: 27542412] (IP) IP
Choe KN, Nicolae CM, Constantin D et al. HUWE1 interacts with PCNA to alleviate replication stress. EMBO Rep. May 4 2016 [PMID: 27146073] (WB, ICC/IF, IP, Human) WB, ICC/IF, IP Human
Rolfo A, Garcia J, Todros T et al. The double life of MULE in preeclamptic and IUGR placentae. Cell Death Dis 2012 May [PMID: 22552282]
Noy T, Suad O, Taglicht D, Ciechanover A. HUWE1 ubiquitinates MyoD and targets it for proteasomal degradation. Biochem Biophys Res Commun;418(2):408-13. 2012 Feb 10. [PMID: 22277673]
Mandel I, Paperna T, Miller A et al. Aberrant expression of the apoptosis-related proteins BAK and MCL1 in T cells in multiple sclerosis. J Neuroimmunol 2012 Mar [PMID: 22257632]
Chanthery YH, Gustafson WC, Itsara M et al. Paracrine signaling through MYCN enhances tumor-vascular interactions in neuroblastoma. Sci Transl Med 2012 Jan [PMID: 22218692]
Markkanen E, van Loon B, Ferrari E et al. Regulation of oxidative DNA damage repair by DNA polymerase ? and MutYH by cross-talk of phosphorylation and ubiquitination. Proc Natl Acad Sci U S A 2012 Jan [PMID: 22203964]
Kon N, Zhong J, Qiang L et al. Inactivation of arf-bp1 induces p53 activation and diabetic phenotypes in mice. J Biol Chem 2012 Feb [PMID: 22187431]
Yamamoto SP, Okawa K, Nakano T et al. Huwe1, a novel cellular interactor of Gag-Pol through integrase binding, negatively influences HIV-1 infectivity Microbes Infect 2011 Apr [PMID: 21167302] (IP, WB, Human) IP, WB Human
Show All 14 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HUWE1 Antibody (NB100-652). (Showing 1 - 1 of 1 FAQs).

  1. Can you tell me what percentage of gel was used for the Western Blot and what the transfer time and conditions were for product NB100-652.
    • This product is tested by a collaborator so I do not have the specifics for this antibody in particular at the moment. I have consulted our lab and for a large protein like this (around 480kDa) we recommend either a 5% or 3-8% gradient polyacrylamide gel. Additionally, we recommend that you boil your samples for 10 minutes at 68 degrees and use your standard transfer conditions. If you need a ladder for that high of a molecular weight, we use HiMark from Invitrogen.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HUWE1
Entrez
Uniprot