HUWE1 Antibody

Images

 
Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody used for WB at 0.1 ...read more
Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiLasu1/Ureb1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.
Immunoprecipitation: HUWE1 Antibody [NB100-652] - Detection of human Lasu1/Ureb1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

HUWE1 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residues 2250 and 2300 of human Upstream Regulatory Element Binding Protein 1 using the numbering given in entry NP_113584.3 (GeneID 10075).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HUWE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:1000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
ICC/IF reactivity reported in (PMID: 27146073). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
481 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-652 in the following applications:

  • 1 publication
  • IP
    4 publications
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31959916).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HUWE1 Antibody

  • arf-bp1
  • EC 6.3.2
  • EC 6.3.2.6
  • HECT, UBA and WWE domain containing 1
  • HECTH9
  • HSPC272
  • KIAA0312MULE
  • Mcl-1 ubiquitin ligase E3
  • Mule
  • UREB1UBA and WWE domain-containing protein 1
  • URE-binding protein 1

Background

ARFBP1 is a novel E3 ubiquitin ligase. It interacts with p19ARF and its ligase activity is inhibited by this interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for HUWE1 Antibody (NB100-652)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(4)
WB
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-652 Applications Species
Yang SW, Huang X, Lin W et al. Structural basis for substrate recognition and chemical inhibition of oncogenic MAGE ubiquitin ligases Nat Commun Oct 1 2020 [PMID: 33004795] (WB, Human) WB Human
Yang SW, Li L, Connelly JP et al. A Cancer-Specific Ubiquitin Ligase Drives mRNA Alternative Polyadenylation by Ubiquitinating the mRNA 3' End Processing Complex Mol. Cell Jan 8 2020 [PMID: 31980388]
Xu YM, Gao Q, Zhang JZ et al. Prolyl hydroxylase 3 controls the intestine goblet cell generation through stabilizing ATOH1 Cell Death Differ. Jan 20 2020 [PMID: 31959916] (WB, IP, Human, Mouse) WB, IP Human, Mouse
Suzuki T, Okamoto T, Katoh H et al. Infection with flaviviruses requires BCLXL for cell survival. PLoS Pathog. Sep 27 2018 [PMID: 30261081] (WB, Human) WB Human
Crow MS, Cristea IM. Human antiviral protein IFIX suppresses viral gene expression during HSV-1 infection and is counteracted by virus-induced proteasomal degradation Mol. Cell Proteomics Jan 11 2017 [PMID: 28077445]
Cheng YF, Tong M, Edge AS. Destabilization of Atoh1 by E3 Ubiquitin Ligase Huwe1 and Casein Kinase 1 Is Essential for Normal Sensory Hair Cell Development. J. Biol. Chem. Aug 19 2016 [PMID: 27542412] (IP) IP
Choe KN, Nicolae CM, Constantin D et al. HUWE1 interacts with PCNA to alleviate replication stress. EMBO Rep. May 4 2016 [PMID: 27146073] (WB, ICC/IF, IP, Human) WB, ICC/IF, IP Human
Rolfo A, Garcia J, Todros T et al. The double life of MULE in preeclamptic and IUGR placentae. Cell Death Dis 2012 May [PMID: 22552282]
Noy T, Suad O, Taglicht D, Ciechanover A. HUWE1 ubiquitinates MyoD and targets it for proteasomal degradation. Biochem Biophys Res Commun;418(2):408-13. 2012 Feb 10. [PMID: 22277673]
Mandel I, Paperna T, Miller A et al. Aberrant expression of the apoptosis-related proteins BAK and MCL1 in T cells in multiple sclerosis. J Neuroimmunol 2012 Mar [PMID: 22257632]
Show All 18 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HUWE1 Antibody (NB100-652). (Showing 1 - 1 of 1 FAQs).

  1. Can you tell me what percentage of gel was used for the Western Blot and what the transfer time and conditions were for product NB100-652.
    • This product is tested by a collaborator so I do not have the specifics for this antibody in particular at the moment. I have consulted our lab and for a large protein like this (around 480kDa) we recommend either a 5% or 3-8% gradient polyacrylamide gel. Additionally, we recommend that you boil your samples for 10 minutes at 68 degrees and use your standard transfer conditions. If you need a ladder for that high of a molecular weight, we use HiMark from Invitrogen.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-652AF350
Alexa Fluor 405 NB100-652AF405
Alexa Fluor 488 NB100-652AF488
Alexa Fluor 532 NB100-652AF532
Alexa Fluor 594 NB100-652AF594
Alexa Fluor 647 NB100-652AF647
Alexa Fluor 700 NB100-652AF700
Alexa Fluor 750 NB100-652AF750
Biotin NB100-652B
DyLight 350 NB100-652UV
DyLight 405 NB100-652V
DyLight 488 NB100-652G
DyLight 550 NB100-652R
DyLight 594 NB100-652DL594
DyLight 650 NB100-652C
DyLight 680 NB100-652FR
DyLight 755 NB100-652IR
FITC NB100-652F
HRP NB100-652H
Janelia Fluor 549 NB100-652JF549
Janelia Fluor 646 NB100-652JF646

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Bioinformatics

Gene Symbol HUWE1
Entrez
Uniprot