Isopeptidase T/USP5 Antibody

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Independent Antibodies: Western Blot: Isopeptidase T/USP5 Antibody [NB100-77285] - Detection of Human and Mouse USP5/IsoT on HeLa whole cell lysate using NB100-77285. USP5/IsoT was also immunoprecipitated by ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Isopeptidase T/USP5 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human ubiquitin specific peptidase 5 (isopeptidase T) using the numbering given in entry CAA62690.1 (GeneID 8078).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Isopeptidase T/USP5 Overexpression Lysate
Publications
Read Publication using NB100-77285.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Isopeptidase T/USP5 Antibody

  • Deubiquitinating enzyme 5
  • EC 3.1.2.15
  • EC 3.4.19.12
  • Isopeptidase T
  • IsoT
  • ISOTubiquitin carboxyl-terminal hydrolase 5
  • ubiquitin isopeptidase T
  • ubiquitin specific peptidase 5 (isopeptidase T)
  • ubiquitin specific protease 5 (isopeptidase T)
  • ubiquitin thioesterase 5
  • Ubiquitin thiolesterase 5
  • ubiquitin-specific protease-5 (ubiquitin isopeptidase T)
  • Ubiquitin-specific-processing protease 5
  • USP5

Background

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 5 (USP5) is a member of this family. The upregulation of USP5 has been found to be associated with fetal Down syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Isopeptidase T/USP5 Antibody (NB100-77285)(1)


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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol USP5
Entrez
Uniprot