Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free

Western Blot: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5.

Immunohistochemistry: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - Staining of paraffin-embedded Human pancreas tissue using anti-USP5 mouse monoclonal antibody.

Western Blot: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - Analysis of extracts (35ug) from 9 different cell lines by using anti-USP5 monoclonal antibody.

Immunohistochemistry: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-USP5 mouse monoclonal antibody.

Immunohistochemistry: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-USP5 mouse monoclonal antibody.

Immunohistochemistry: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - Staining of paraffin-embedded Human colon tissue using anti-USP5 mouse monoclonal antibody.

Immunohistochemistry: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - Staining of paraffin-embedded Human Kidney tissue using anti-USP5 mouse monoclonal antibody.

Immunohistochemistry: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - Staining of paraffin-embedded Human lung tissue using anti-USP5 mouse monoclonal antibody.

Immunohistochemistry: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - Staining of paraffin-embedded Human thyroid tissue using anti-USP5 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, IHC
• Clone: OTI2E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human USP5 (NP_003472) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-2000
• Theoretical MW: 93.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free

• Deubiquitinating enzyme 5
• EC 3.1.2.15
• EC 3.4.19.12
• Isopeptidase T
• IsoT
• ISOTubiquitin carboxyl-terminal hydrolase 5
• ubiquitin isopeptidase T
• ubiquitin specific peptidase 5 (isopeptidase T)
• ubiquitin specific protease 5 (isopeptidase T)
• ubiquitin thioesterase 5
• Ubiquitin thiolesterase 5
• ubiquitin-specific protease-5 (ubiquitin isopeptidase T)
• Ubiquitin-specific-processing protease 5
• USP5

Background

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 5 (USP5) is a member of this family. The upregulation of USP5 has been found to be associated with fetal Down syndrome. Alternate names for USP include ubiquitin carboxyl-terminal hydrolase 5, ubiquitin thioesterase 5, ubiquitin-specific-processing protease 5, deubuiquitinating enzyme 5, isopeptidase T, and ISOT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP5